This paper investigates the textured surface generation mechanism in vibration-assisted micro milling through modelling and experimental approaches. To decouple the effects of tool geometry and kinematics of vibration-assisted milling, a surface generation model based on homogenous matrices transformation is proposed. On this basis, series of simulations are performed to provide insights into the effects of various vibration parameters (frequency, amplitude and phase difference) on the generation mechanism of typical textured surfaces in 1D and 2D vibration-assisted milling. Furthermore, the wettability tests are performed on the machined surfaces with various surface texture topographies. The fish-scale textured surfaces are found to exhibit better wettability than those with wavy-structure. The results indicate that vibration-assisted milling is an effective method to generate certain surface textures with controllable wettability.