Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology

Polly Hayes, Vladimir Varga, Sofia Olego-Fernandez, Jack Sunter, Michael L. Ginger, Keith Gull

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components.

LanguageEnglish
Pages377-384
Number of pages8
JournalJournal of Cell Biology
Volume206
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

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Trypanosoma brucei brucei
Calpain
Trypanosomiasis
Flagella
Basal Bodies
Cytoskeletal Proteins
Cell Shape
Life Cycle Stages
Cell Size
Parasites
Proteins
Membranes

Cite this

Hayes, Polly ; Varga, Vladimir ; Olego-Fernandez, Sofia ; Sunter, Jack ; Ginger, Michael L. ; Gull, Keith. / Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology. In: Journal of Cell Biology. 2014 ; Vol. 206, No. 3. pp. 377-384.
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Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology. / Hayes, Polly; Varga, Vladimir; Olego-Fernandez, Sofia; Sunter, Jack; Ginger, Michael L.; Gull, Keith.

In: Journal of Cell Biology, Vol. 206, No. 3, 2014, p. 377-384.

Research output: Contribution to journalArticle

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