Molar mass and solution conformation of branched α(1 → 4), α(1 → 6) Glucans. Part I

Glycogens in water

Gordon A. Morris, Shirley Ang, Sandra E. Hill, Susan Lewis, Bernd Schäfer, Ulf Nobbmann, Stephen E. Harding

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Solution molar masses and conformations of glycogens from different sources (rabbit, oyster, mussel and bovine) were analysed using sedimentation velocity in the analytical ultracentrifuge, size-exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS), size-exclusion chromatography coupled to a differential pressure viscometer and dynamic light scattering. Rabbit, oyster and mussel glycogens consisted of one population of high molar mass (weight averages ranging from 4.6 × 106 to 1.1 × 107 g/mol) as demonstrated by sedimentation velocity and SEC-MALLS, whereas bovine glycogen had a bimodal distribution of significantly lower molar mass (1.0 × 105 and 4.5 × 105 g/mol). The spherical structure of all glycogen molecules was demonstrated in the slopes of the Mark-Houwink-Kuhn-Sakurada-type power-law relations for sedimentation coefficient (s20,wo), intrinsic viscosity ([η]), radius of gyration (rg,z) and radius of hydration (rH,z), respectively, and was further supported by the ρ (=rg,z/rH,z) function, the fractal dimension and the Perrin function. The degree of branching was estimated to be ∼10% from the shrinking factors, g′ (=[η]branched/[η]linear) and also h (=(f/fo)branched/(f/fo)linear), respectively, where (f/fo) is the translational frictional ratio, consistent with expectation.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalCarbohydrate Polymers
Volume71
Issue number1
Early online date29 May 2007
DOIs
Publication statusPublished - 5 Jan 2008
Externally publishedYes

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Glucans
Molar mass
Glycogen
Conformations
Size exclusion chromatography
Sedimentation
Water
Light scattering
Viscometers
Lasers
Dynamic light scattering
Fractal dimension
Hydration
Viscosity
Molecules

Cite this

Morris, Gordon A. ; Ang, Shirley ; Hill, Sandra E. ; Lewis, Susan ; Schäfer, Bernd ; Nobbmann, Ulf ; Harding, Stephen E. / Molar mass and solution conformation of branched α(1 → 4), α(1 → 6) Glucans. Part I : Glycogens in water. In: Carbohydrate Polymers. 2008 ; Vol. 71, No. 1. pp. 101-108.
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Molar mass and solution conformation of branched α(1 → 4), α(1 → 6) Glucans. Part I : Glycogens in water. / Morris, Gordon A.; Ang, Shirley; Hill, Sandra E.; Lewis, Susan; Schäfer, Bernd; Nobbmann, Ulf; Harding, Stephen E.

In: Carbohydrate Polymers, Vol. 71, No. 1, 05.01.2008, p. 101-108.

Research output: Contribution to journalArticle

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