Molecular biological detection of anaerobic gut fungi (Neocallimastigales) from landfill sites

Robert J. Lockhart, Michele I. Van Dyke, Ian R. Beadle, Paul Humphreys, Alan J. McCarthy

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.

Original languageEnglish
Pages (from-to)5659-5661
Number of pages3
JournalApplied and Environmental Microbiology
Volume72
Issue number8
DOIs
Publication statusPublished - Aug 2006

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