Molecular genetic differentiation in earthworms inhabiting a heterogeneous Pb-polluted landscape

J. Andre, R.A. King, S.r. Stürzenbaum, P. Kille, M.E. Hodson, A.J. Morgan

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages (‘A’ and ‘B’), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central ‘ecological island’ site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies.
LanguageEnglish
Pages883-890
Number of pages8
JournalEnvironmental Pollution
Volume158
Issue number3
Early online date8 Oct 2009
DOIs
Publication statusPublished - 1 Mar 2010
Externally publishedYes

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Oligochaeta
Mitochondrial DNA
Islands
Molecular Biology
Genes
Bioinformatics
Amplified Fragment Length Polymorphism Analysis
Assays
Phosphates
Fourier Transform Infrared Spectroscopy
Computational Biology
Proteins
Soils
Libraries
Soil
Technology
Mutation

Cite this

Andre, J. ; King, R.A. ; Stürzenbaum, S.r. ; Kille, P. ; Hodson, M.E. ; Morgan, A.J. / Molecular genetic differentiation in earthworms inhabiting a heterogeneous Pb-polluted landscape. In: Environmental Pollution. 2010 ; Vol. 158, No. 3. pp. 883-890.
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Molecular genetic differentiation in earthworms inhabiting a heterogeneous Pb-polluted landscape. / Andre, J.; King, R.A.; Stürzenbaum, S.r.; Kille, P.; Hodson, M.E.; Morgan, A.J.

In: Environmental Pollution, Vol. 158, No. 3, 01.03.2010, p. 883-890.

Research output: Contribution to journalArticle

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AU - King, R.A.

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AB - A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages (‘A’ and ‘B’), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central ‘ecological island’ site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies.

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