Molecular Weight Distribution Evaluation of Polysaccharides and Glycoconjugates Using Analytical Ultracentrifugation

Stephen E. Harding, Ali Saber Abdelhameed, Gordon A. Morris

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

We review here the advances that have been made in methodology using the analytical ultracentrifuge for characterising polydisperse polymer systems with a quasi-continuous distribution of molecular weight. These advances include improved ways of defining the weight and z-average molecular weights of a distribution from sedimentation equilibrium experiments and hence the ratio Mz=Mw, off-line calibration of size exclusion chromatography by sedimentation equilibrium and conversion of a sedimentation coefficient distribution into a molecular weight distribution. Although these methods are applicable to other polymeric systems, we focus on polysaccharides, mucins and other glycoconjugates, systems which particularly benefit from the availability of recently available long optical path length cells for the minimisation of complications through thermodynamic non-ideality.

LanguageEnglish
Pages714-720
Number of pages7
JournalMacromolecular Bioscience
Volume10
Issue number7
DOIs
Publication statusPublished - 7 Jul 2010
Externally publishedYes

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Glycoconjugates
Ultracentrifugation
Molecular weight distribution
Polysaccharides
Sedimentation
Molecular Weight
Molecular weight
Size exclusion chromatography
Mucins
Thermodynamics
Calibration
Gel Chromatography
Polymers
Availability
Weights and Measures
Experiments

Cite this

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Molecular Weight Distribution Evaluation of Polysaccharides and Glycoconjugates Using Analytical Ultracentrifugation. / Harding, Stephen E.; Abdelhameed, Ali Saber; Morris, Gordon A.

In: Macromolecular Bioscience, Vol. 10, No. 7, 07.07.2010, p. 714-720.

Research output: Contribution to journalReview article

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