Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low-Molecular-Weight Analytes

New composite layer architecture of 3D hydrogel polymer network that is loaded with molecularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low-molecular-weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding-matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a label-free readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photocrosslinkable poly( N-isopropylacrylamide)-based hydrogel and poly[(ethylene glycol dimethylacrylate)-(methacrylic acid)] nanoparticles that are imprinted with L-Boc-phenylalanine-anilide ( L-BFA, molecular weight 353 g mol^-1). Titration experiments with the specific target and other structurally similar reference compounds show good specificity and limit of detection for target L-BFA as low as 2 × 10^-6 M. (Figure Presented).