TY - JOUR
T1 - Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis
AU - Brüning, A
AU - Phipps, P
AU - Posnett, E
AU - Canning, E U
PY - 1997/1
Y1 - 1997/1
N2 - The production of monoclonal antibodies to the bloodstages of the haemoprotozoan parasites Babesia caballi and Babesia equi and the characterization of their corresponding antigens are described. Species specific and immunogenic proteins of both parasites were identified using SDS-PAGE, Western blotting and ELISA. These proteins were then electroeluted from SDS-PAGE gels and used to immunize BALB/c mice for hybridoma production. One monoclonal antibody (Mab), designated BC5.37.70.27 (BC5), recognized a 70 kDa protein of B. caballi as demonstrated by Western blotting under reducing conditions. Another Mab, BE1.24/2.95 (BEI), recognized a 34 kDa protein of B. equi. Both Mabs reacted specifically in indirect ELISA when isolated whole merozoites were used as antigen. Preliminary studies using the two Mabs in a competitive ELISA (cELISA) suggest that the cELISA for the detection of B. caballi infection is more sensitive than the commonly used complement fixation test but that refinement is necessary for the B. equi system.
AB - The production of monoclonal antibodies to the bloodstages of the haemoprotozoan parasites Babesia caballi and Babesia equi and the characterization of their corresponding antigens are described. Species specific and immunogenic proteins of both parasites were identified using SDS-PAGE, Western blotting and ELISA. These proteins were then electroeluted from SDS-PAGE gels and used to immunize BALB/c mice for hybridoma production. One monoclonal antibody (Mab), designated BC5.37.70.27 (BC5), recognized a 70 kDa protein of B. caballi as demonstrated by Western blotting under reducing conditions. Another Mab, BE1.24/2.95 (BEI), recognized a 34 kDa protein of B. equi. Both Mabs reacted specifically in indirect ELISA when isolated whole merozoites were used as antigen. Preliminary studies using the two Mabs in a competitive ELISA (cELISA) suggest that the cELISA for the detection of B. caballi infection is more sensitive than the commonly used complement fixation test but that refinement is necessary for the B. equi system.
KW - Animals
KW - Antibodies, Monoclonal
KW - Antibodies, Protozoan
KW - Antibody Specificity
KW - Antigens, Protozoan/analysis
KW - Babesia/immunology
KW - Babesiosis/diagnosis
KW - Electrophoresis, Polyacrylamide Gel
KW - Enzyme-Linked Immunosorbent Assay
KW - Horses
KW - Mice
KW - Mice, Inbred BALB C/immunology
KW - Sensitivity and Specificity
KW - Serologic Tests
U2 - 10.1016/S0304-4017(96)01074-6
DO - 10.1016/S0304-4017(96)01074-6
M3 - Article
C2 - 9066047
VL - 68
SP - 11
EP - 26
JO - Veterinary Parasitology: Regional Studies and Reports
JF - Veterinary Parasitology: Regional Studies and Reports
SN - 0304-4017
IS - 1-2
ER -