Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis

A Brüning, P Phipps, E Posnett, E U Canning

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The production of monoclonal antibodies to the bloodstages of the haemoprotozoan parasites Babesia caballi and Babesia equi and the characterization of their corresponding antigens are described. Species specific and immunogenic proteins of both parasites were identified using SDS-PAGE, Western blotting and ELISA. These proteins were then electroeluted from SDS-PAGE gels and used to immunize BALB/c mice for hybridoma production. One monoclonal antibody (Mab), designated BC5.37.70.27 (BC5), recognized a 70 kDa protein of B. caballi as demonstrated by Western blotting under reducing conditions. Another Mab, BE1.24/2.95 (BEI), recognized a 34 kDa protein of B. equi. Both Mabs reacted specifically in indirect ELISA when isolated whole merozoites were used as antigen. Preliminary studies using the two Mabs in a competitive ELISA (cELISA) suggest that the cELISA for the detection of B. caballi infection is more sensitive than the commonly used complement fixation test but that refinement is necessary for the B. equi system.

LanguageEnglish
Pages11-26
Number of pages16
JournalVeterinary Parasitology
Volume68
Issue number1-2
DOIs
Publication statusPublished - Jan 1997
Externally publishedYes

Fingerprint

Babesia caballi
Theileria equi
Babesia
serodiagnosis
Serologic Tests
Horses
monoclonal antibodies
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
enzyme-linked immunosorbent assay
Polyacrylamide Gel Electrophoresis
Parasites
Western blotting
proteins
Western Blotting
antigens
Merozoites
Antigens
parasites
Complement Fixation Tests

Cite this

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title = "Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis",
abstract = "The production of monoclonal antibodies to the bloodstages of the haemoprotozoan parasites Babesia caballi and Babesia equi and the characterization of their corresponding antigens are described. Species specific and immunogenic proteins of both parasites were identified using SDS-PAGE, Western blotting and ELISA. These proteins were then electroeluted from SDS-PAGE gels and used to immunize BALB/c mice for hybridoma production. One monoclonal antibody (Mab), designated BC5.37.70.27 (BC5), recognized a 70 kDa protein of B. caballi as demonstrated by Western blotting under reducing conditions. Another Mab, BE1.24/2.95 (BEI), recognized a 34 kDa protein of B. equi. Both Mabs reacted specifically in indirect ELISA when isolated whole merozoites were used as antigen. Preliminary studies using the two Mabs in a competitive ELISA (cELISA) suggest that the cELISA for the detection of B. caballi infection is more sensitive than the commonly used complement fixation test but that refinement is necessary for the B. equi system.",
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author = "A Br{\"u}ning and P Phipps and E Posnett and Canning, {E U}",
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Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis. / Brüning, A; Phipps, P; Posnett, E; Canning, E U.

In: Veterinary Parasitology, Vol. 68, No. 1-2, 01.1997, p. 11-26.

Research output: Contribution to journalArticle

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AU - Phipps, P

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AU - Canning, E U

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AB - The production of monoclonal antibodies to the bloodstages of the haemoprotozoan parasites Babesia caballi and Babesia equi and the characterization of their corresponding antigens are described. Species specific and immunogenic proteins of both parasites were identified using SDS-PAGE, Western blotting and ELISA. These proteins were then electroeluted from SDS-PAGE gels and used to immunize BALB/c mice for hybridoma production. One monoclonal antibody (Mab), designated BC5.37.70.27 (BC5), recognized a 70 kDa protein of B. caballi as demonstrated by Western blotting under reducing conditions. Another Mab, BE1.24/2.95 (BEI), recognized a 34 kDa protein of B. equi. Both Mabs reacted specifically in indirect ELISA when isolated whole merozoites were used as antigen. Preliminary studies using the two Mabs in a competitive ELISA (cELISA) suggest that the cELISA for the detection of B. caballi infection is more sensitive than the commonly used complement fixation test but that refinement is necessary for the B. equi system.

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