TY - JOUR
T1 - Moxifloxacin-loaded electrospun polymeric composite nanofibers-based wound dressing for enhanced antibacterial activity and healing efficacy
AU - Hameed , Misbah
AU - Rasul , Akhtar
AU - Nazir, Ahsan
AU - Yousaf, Abid
AU - Hussain, Talib
AU - Khan , Ikram Ullah
AU - Abbas , Ghulam
AU - Asif , Sharjeel
AU - Yousaf, Qurrat ul Ain
AU - Ghori, Muhammad Usman
AU - Shahzad, Yasser
N1 - Funding Information:
No financial support was available for this research work. The authors are obliged to Government College University Faisalabad and National Textile University Faisalabad for providing all the chemicals and the laboratory facility.
Publisher Copyright:
© 2020, © 2020 Taylor & Francis Group, LLC.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/9/1
Y1 - 2021/9/1
N2 - Nanofibers were prepared with different ratios of chitosan (CS) and polyethylene oxide (PEO) via the electrospinning technique, and tested for morphological features using scanning electron microscopy (SEM). Drug content, drug loading, release of moxifloxacin (MXF) at pH 4.7 and pH 5.5, and degree of swelling were investigated. Further characterization was accomplished via X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial activity was studied in comparison with blank nanofibers. Furthermore, wound healing was studied in Sprague-Dawley rats. A formulation consisting of CS/PEO (50/50) exhibited smooth-surfaced and beadless nanofibers having a mean diameter of 138 ± 25 nm. Drug content and drug loading in the optimized formulation, consisting of MXF, CS and PEO (0.5/2.5/2.5, w/w/w), were 99–101% and 9.1%, respectively. MXF release in 48 hours was 79.83 ± 4.2% and 94.29 ± 3.9% at pH 7.4 and pH 5.5, respectively. Moreover, degree of swelling was 133 ± 13%. The drug existed in the amorphous state and had no covalent interaction with the polymers. MXF-loaded nanofibers demonstrated greater stability, antibacterial activity and wound healing efficacy than did blank nanofibers. Accordingly, MXF-loaded CS-PEO polymeric composite nanofibers might be a potential wound dressing for effective wound healing.
AB - Nanofibers were prepared with different ratios of chitosan (CS) and polyethylene oxide (PEO) via the electrospinning technique, and tested for morphological features using scanning electron microscopy (SEM). Drug content, drug loading, release of moxifloxacin (MXF) at pH 4.7 and pH 5.5, and degree of swelling were investigated. Further characterization was accomplished via X-ray diffraction (XRD), thermogravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial activity was studied in comparison with blank nanofibers. Furthermore, wound healing was studied in Sprague-Dawley rats. A formulation consisting of CS/PEO (50/50) exhibited smooth-surfaced and beadless nanofibers having a mean diameter of 138 ± 25 nm. Drug content and drug loading in the optimized formulation, consisting of MXF, CS and PEO (0.5/2.5/2.5, w/w/w), were 99–101% and 9.1%, respectively. MXF release in 48 hours was 79.83 ± 4.2% and 94.29 ± 3.9% at pH 7.4 and pH 5.5, respectively. Moreover, degree of swelling was 133 ± 13%. The drug existed in the amorphous state and had no covalent interaction with the polymers. MXF-loaded nanofibers demonstrated greater stability, antibacterial activity and wound healing efficacy than did blank nanofibers. Accordingly, MXF-loaded CS-PEO polymeric composite nanofibers might be a potential wound dressing for effective wound healing.
KW - Antibacterial
KW - Electrospinning
KW - Moxifloxacin
KW - Polymeric nanofibers
KW - Wound dressing
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85087810857&partnerID=8YFLogxK
U2 - 10.1080/00914037.2020.1785464
DO - 10.1080/00914037.2020.1785464
M3 - Article
VL - 70
SP - 1271
EP - 1279
JO - International Journal of Polymeric Materials and Polymeric Biomaterials
JF - International Journal of Polymeric Materials and Polymeric Biomaterials
SN - 0091-4037
IS - 17
ER -
