Nanoscale crystallinity modulates cell proliferation on plasma sprayed surfaces

Alan M. Smith, Jennifer Z. Paxton, Yi Pei Hung, Martin J. Hadley, James Bowen, Richard L. Williams, Liam M. Grover

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Calcium phosphate coatings have been applied to the surface of metallic prostheses to mediate hard and soft tissue attachment for more than 40 years. Most coatings are formed of high purity hydroxyapatite, and coating methods are often designed to produce highly crystalline surfaces. It is likely however, that coatings of lower crystallinity can facilitate more rapid tissue attachment since the surface will exhibit a higher specific surface area and will be considerably more reactive than a comparable highly crystalline surface. Here we test this hypothesis by growing a population of MC3T3 osteoblast-like cells on the surface of two types of hip prosthesis with similar composition, but with differing crystallinity. The surfaces with lower crystallinity facilitated more rapid cell attachment and increased proliferation rate, despite having a less heterogeneous surface topography. This work highlights that the influence of the crystallinity of HA at the nano-scale is dominant over macroscale topography for cell adhesion and growth. Furthermore, crystallinity could be easily adjusted by without compromising coating purity. These findings could facilitate designing novel coated calcium phosphate surfaces that more rapidly bond tissue following implantation.

Original languageEnglish
Pages (from-to)5-10
Number of pages6
JournalMaterials Science and Engineering C
Volume48
DOIs
Publication statusPublished - 1 Mar 2015

Fingerprint

Cell proliferation
crystallinity
Plasmas
Coatings
Calcium phosphate
Tissue
attachment
coatings
calcium phosphates
Crystalline materials
Hip prostheses
Phosphate coatings
topography
purity
cells
Osteoblasts
Cell adhesion
Cell growth
Surface topography
Durapatite

Cite this

Smith, Alan M. ; Paxton, Jennifer Z. ; Hung, Yi Pei ; Hadley, Martin J. ; Bowen, James ; Williams, Richard L. ; Grover, Liam M. / Nanoscale crystallinity modulates cell proliferation on plasma sprayed surfaces. In: Materials Science and Engineering C. 2015 ; Vol. 48. pp. 5-10.
@article{5d86aee8cc31473ba11627d62fa875c1,
title = "Nanoscale crystallinity modulates cell proliferation on plasma sprayed surfaces",
abstract = "Calcium phosphate coatings have been applied to the surface of metallic prostheses to mediate hard and soft tissue attachment for more than 40 years. Most coatings are formed of high purity hydroxyapatite, and coating methods are often designed to produce highly crystalline surfaces. It is likely however, that coatings of lower crystallinity can facilitate more rapid tissue attachment since the surface will exhibit a higher specific surface area and will be considerably more reactive than a comparable highly crystalline surface. Here we test this hypothesis by growing a population of MC3T3 osteoblast-like cells on the surface of two types of hip prosthesis with similar composition, but with differing crystallinity. The surfaces with lower crystallinity facilitated more rapid cell attachment and increased proliferation rate, despite having a less heterogeneous surface topography. This work highlights that the influence of the crystallinity of HA at the nano-scale is dominant over macroscale topography for cell adhesion and growth. Furthermore, crystallinity could be easily adjusted by without compromising coating purity. These findings could facilitate designing novel coated calcium phosphate surfaces that more rapidly bond tissue following implantation.",
keywords = "Calcium phosphate, Coating, Crystallinity, Hydroxyapatite, Prosthesis",
author = "Smith, {Alan M.} and Paxton, {Jennifer Z.} and Hung, {Yi Pei} and Hadley, {Martin J.} and James Bowen and Williams, {Richard L.} and Grover, {Liam M.}",
year = "2015",
month = "3",
day = "1",
doi = "10.1016/j.msec.2014.11.006",
language = "English",
volume = "48",
pages = "5--10",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

Nanoscale crystallinity modulates cell proliferation on plasma sprayed surfaces. / Smith, Alan M.; Paxton, Jennifer Z.; Hung, Yi Pei; Hadley, Martin J.; Bowen, James; Williams, Richard L.; Grover, Liam M.

In: Materials Science and Engineering C, Vol. 48, 01.03.2015, p. 5-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nanoscale crystallinity modulates cell proliferation on plasma sprayed surfaces

AU - Smith, Alan M.

AU - Paxton, Jennifer Z.

AU - Hung, Yi Pei

AU - Hadley, Martin J.

AU - Bowen, James

AU - Williams, Richard L.

AU - Grover, Liam M.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Calcium phosphate coatings have been applied to the surface of metallic prostheses to mediate hard and soft tissue attachment for more than 40 years. Most coatings are formed of high purity hydroxyapatite, and coating methods are often designed to produce highly crystalline surfaces. It is likely however, that coatings of lower crystallinity can facilitate more rapid tissue attachment since the surface will exhibit a higher specific surface area and will be considerably more reactive than a comparable highly crystalline surface. Here we test this hypothesis by growing a population of MC3T3 osteoblast-like cells on the surface of two types of hip prosthesis with similar composition, but with differing crystallinity. The surfaces with lower crystallinity facilitated more rapid cell attachment and increased proliferation rate, despite having a less heterogeneous surface topography. This work highlights that the influence of the crystallinity of HA at the nano-scale is dominant over macroscale topography for cell adhesion and growth. Furthermore, crystallinity could be easily adjusted by without compromising coating purity. These findings could facilitate designing novel coated calcium phosphate surfaces that more rapidly bond tissue following implantation.

AB - Calcium phosphate coatings have been applied to the surface of metallic prostheses to mediate hard and soft tissue attachment for more than 40 years. Most coatings are formed of high purity hydroxyapatite, and coating methods are often designed to produce highly crystalline surfaces. It is likely however, that coatings of lower crystallinity can facilitate more rapid tissue attachment since the surface will exhibit a higher specific surface area and will be considerably more reactive than a comparable highly crystalline surface. Here we test this hypothesis by growing a population of MC3T3 osteoblast-like cells on the surface of two types of hip prosthesis with similar composition, but with differing crystallinity. The surfaces with lower crystallinity facilitated more rapid cell attachment and increased proliferation rate, despite having a less heterogeneous surface topography. This work highlights that the influence of the crystallinity of HA at the nano-scale is dominant over macroscale topography for cell adhesion and growth. Furthermore, crystallinity could be easily adjusted by without compromising coating purity. These findings could facilitate designing novel coated calcium phosphate surfaces that more rapidly bond tissue following implantation.

KW - Calcium phosphate

KW - Coating

KW - Crystallinity

KW - Hydroxyapatite

KW - Prosthesis

UR - http://www.scopus.com/inward/record.url?scp=84911876630&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2014.11.006

DO - 10.1016/j.msec.2014.11.006

M3 - Article

VL - 48

SP - 5

EP - 10

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -