Abstract
Electrophoretic mobility shift assays revealed minimal levels of NF-κB activity in rat distal lung epithelial cells cultured at fetal (23 mmHg) or adult alveolar (100 mmHg) PO2, but revealed significant activation of this transcription factor in cells exposed to a rise in PO2 mimicking that experienced at birth. This response was entirely abolished by pretreating cells with 5 mM sulfasalazine (SSA). This shift in PO2 also evoked a rise in apical Na+ conductance (GNa+) that may underlie the O2-evoked stimulation of Na+ transport seen in these cells. Pretreatment with SSA had no effect upon GNa+ in cells cultured continually at adult or fetal PO2 but did inhibit the increase in GNa+ seen in cells that had experienced the rise in PO2. O2-evoked activation of NF-κB may thus mediate the increased Na+ transport that occurs when the distal lung epithelial cells are exposed to a physiologically-relevant increase in PO2.
Original language | English |
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Pages (from-to) | 987-992 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 281 |
Issue number | 4 |
DOIs | |
Publication status | Published - 9 Mar 2001 |
Externally published | Yes |