Novel effect of plant lectins on the inhibition of streptococcus mutans biofilm formation on saliva-coated surface

B. Islam, S. N. Khan, A. Naeem, V. Sharma, A. U. Khan

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Aims: Dental caries is caused by the disturbance in oral homeostasis, marked by a notable increase in the population of Streptococcus mutans. Lectins are a group of plant proteins that are capable of recognizing the glycoconjugates present on the bacterial surface. The aim of this study was to evaluate the effect of seven plant lectins on the growth and initial adhesion of S. mutans. Methods and Results: Lectins of different carbohydrate specificities were isolated from plant sources by conventional methods of protein purification. The effect on growth of S. mutans was evaluated following CLSI guidelines. None of the lectins used in this study inhibited the bacterial growth and multiplication. The adherence and biofilm formation of bacteria to saliva-coated polystyrene plates was tested in the presence of plant lectins. All the plant lectins tested, inhibited both the adherence and biofilm in a concentration dependent manner. Confocal microscopy and scanning electron microscopy were employed to assess the biofilm formation in the presence of plant lectin (glucose/mannose-specific) at sub-minimal inhibitory concentrations. These evaluations revealed that lectins inhibited the clumping and attachment of S. mutans. Conclusions: Lectins tested here inhibited initial biofilm formation by S. mutans. Glucose/Mannose-specific lectin altered the adhesion arrangement of the bacteria on the saliva-coated surfaces. Significance and Impact of the Study: The plant lectins used in this study may offer a novel strategy to reduce development of dental caries by inhibiting the initial adhesion and subsequent biofilm formation of S. mutans.

Original languageEnglish
Pages (from-to)1682-1689
Number of pages8
JournalJournal of Applied Microbiology
Volume106
Issue number5
Early online date6 Apr 2009
DOIs
Publication statusPublished - 1 May 2009
Externally publishedYes

Fingerprint

Plant Lectins
Streptococcus mutans
Biofilms
Saliva
Lectins
Dental Caries
Mannose-Binding Lectins
Growth
Bacteria
Glucose
Plant Proteins
Glycoconjugates
Polystyrenes
Mannose
Confocal Microscopy
Electron Scanning Microscopy
Homeostasis
Carbohydrates
Guidelines
Population

Cite this

@article{d43a3f304cef42628150e42d8cf6a432,
title = "Novel effect of plant lectins on the inhibition of streptococcus mutans biofilm formation on saliva-coated surface",
abstract = "Aims: Dental caries is caused by the disturbance in oral homeostasis, marked by a notable increase in the population of Streptococcus mutans. Lectins are a group of plant proteins that are capable of recognizing the glycoconjugates present on the bacterial surface. The aim of this study was to evaluate the effect of seven plant lectins on the growth and initial adhesion of S. mutans. Methods and Results: Lectins of different carbohydrate specificities were isolated from plant sources by conventional methods of protein purification. The effect on growth of S. mutans was evaluated following CLSI guidelines. None of the lectins used in this study inhibited the bacterial growth and multiplication. The adherence and biofilm formation of bacteria to saliva-coated polystyrene plates was tested in the presence of plant lectins. All the plant lectins tested, inhibited both the adherence and biofilm in a concentration dependent manner. Confocal microscopy and scanning electron microscopy were employed to assess the biofilm formation in the presence of plant lectin (glucose/mannose-specific) at sub-minimal inhibitory concentrations. These evaluations revealed that lectins inhibited the clumping and attachment of S. mutans. Conclusions: Lectins tested here inhibited initial biofilm formation by S. mutans. Glucose/Mannose-specific lectin altered the adhesion arrangement of the bacteria on the saliva-coated surfaces. Significance and Impact of the Study: The plant lectins used in this study may offer a novel strategy to reduce development of dental caries by inhibiting the initial adhesion and subsequent biofilm formation of S. mutans.",
keywords = "Anti-biofilm, CLSM, Plant lectins, SEM, Streptococcus mutans, TFA lectin",
author = "B. Islam and Khan, {S. N.} and A. Naeem and V. Sharma and Khan, {A. U.}",
year = "2009",
month = "5",
day = "1",
doi = "10.1111/j.1365-2672.2008.04135.x",
language = "English",
volume = "106",
pages = "1682--1689",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley",
number = "5",

}

Novel effect of plant lectins on the inhibition of streptococcus mutans biofilm formation on saliva-coated surface. / Islam, B.; Khan, S. N.; Naeem, A.; Sharma, V.; Khan, A. U.

In: Journal of Applied Microbiology, Vol. 106, No. 5, 01.05.2009, p. 1682-1689.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Novel effect of plant lectins on the inhibition of streptococcus mutans biofilm formation on saliva-coated surface

AU - Islam, B.

AU - Khan, S. N.

AU - Naeem, A.

AU - Sharma, V.

AU - Khan, A. U.

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Aims: Dental caries is caused by the disturbance in oral homeostasis, marked by a notable increase in the population of Streptococcus mutans. Lectins are a group of plant proteins that are capable of recognizing the glycoconjugates present on the bacterial surface. The aim of this study was to evaluate the effect of seven plant lectins on the growth and initial adhesion of S. mutans. Methods and Results: Lectins of different carbohydrate specificities were isolated from plant sources by conventional methods of protein purification. The effect on growth of S. mutans was evaluated following CLSI guidelines. None of the lectins used in this study inhibited the bacterial growth and multiplication. The adherence and biofilm formation of bacteria to saliva-coated polystyrene plates was tested in the presence of plant lectins. All the plant lectins tested, inhibited both the adherence and biofilm in a concentration dependent manner. Confocal microscopy and scanning electron microscopy were employed to assess the biofilm formation in the presence of plant lectin (glucose/mannose-specific) at sub-minimal inhibitory concentrations. These evaluations revealed that lectins inhibited the clumping and attachment of S. mutans. Conclusions: Lectins tested here inhibited initial biofilm formation by S. mutans. Glucose/Mannose-specific lectin altered the adhesion arrangement of the bacteria on the saliva-coated surfaces. Significance and Impact of the Study: The plant lectins used in this study may offer a novel strategy to reduce development of dental caries by inhibiting the initial adhesion and subsequent biofilm formation of S. mutans.

AB - Aims: Dental caries is caused by the disturbance in oral homeostasis, marked by a notable increase in the population of Streptococcus mutans. Lectins are a group of plant proteins that are capable of recognizing the glycoconjugates present on the bacterial surface. The aim of this study was to evaluate the effect of seven plant lectins on the growth and initial adhesion of S. mutans. Methods and Results: Lectins of different carbohydrate specificities were isolated from plant sources by conventional methods of protein purification. The effect on growth of S. mutans was evaluated following CLSI guidelines. None of the lectins used in this study inhibited the bacterial growth and multiplication. The adherence and biofilm formation of bacteria to saliva-coated polystyrene plates was tested in the presence of plant lectins. All the plant lectins tested, inhibited both the adherence and biofilm in a concentration dependent manner. Confocal microscopy and scanning electron microscopy were employed to assess the biofilm formation in the presence of plant lectin (glucose/mannose-specific) at sub-minimal inhibitory concentrations. These evaluations revealed that lectins inhibited the clumping and attachment of S. mutans. Conclusions: Lectins tested here inhibited initial biofilm formation by S. mutans. Glucose/Mannose-specific lectin altered the adhesion arrangement of the bacteria on the saliva-coated surfaces. Significance and Impact of the Study: The plant lectins used in this study may offer a novel strategy to reduce development of dental caries by inhibiting the initial adhesion and subsequent biofilm formation of S. mutans.

KW - Anti-biofilm

KW - CLSM

KW - Plant lectins

KW - SEM

KW - Streptococcus mutans

KW - TFA lectin

UR - http://www.scopus.com/inward/record.url?scp=64349091036&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2672.2008.04135.x

DO - 10.1111/j.1365-2672.2008.04135.x

M3 - Article

VL - 106

SP - 1682

EP - 1689

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

ER -