Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs

James R. Boyne; Adrian Whitehouse

doi:10.1016/j.febslet.2009.10.040

Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs

James R. Boyne, Adrian Whitehouse

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, the ability of ORF57 to export mRNA was assessed in cells where the nucleolus was disrupted using Actinomycin D or DRB. We show that rapid disorganisation of the nucleolus was accompanied by a reduction in intronless virus mRNA export, suggesting that an intact nucleolus is essential for efficient KSHV ORF57-mediated intronless mRNA export. Structured summary: MINT-7291377: Aly (uniprotkb:Q86V81) physically interacts (MI:0915) with ORF57 (uniprotkb:Q2HR75) by pull down (MI:0096). MINT-7291330, MINT-7291320: Nucleolin (uniprotkb:P19338) and ORF57 (uniprotkb:Q2HR75) colocalise (MI:0403) by fluorescence microscopy (MI:0416). MINT-7291715: ORF57 (uniprotkb:Q2HR75) and Aly (uniprotkb:Q86V81) colocalise (MI:0403) by fluorescence microscopy (MI:0416).

Original language	English
Pages (from-to)	3549-3556
Number of pages	8
Journal	FEBS Letters
Volume	583
Issue number	22
DOIs	https://doi.org/10.1016/j.febslet.2009.10.040
Publication status	Published - 19 Nov 2009
Externally published	Yes

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@article{9ce229d616c4455db3217791cb3facfd,

title = "Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs",

abstract = "Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, the ability of ORF57 to export mRNA was assessed in cells where the nucleolus was disrupted using Actinomycin D or DRB. We show that rapid disorganisation of the nucleolus was accompanied by a reduction in intronless virus mRNA export, suggesting that an intact nucleolus is essential for efficient KSHV ORF57-mediated intronless mRNA export. Structured summary: MINT-7291377: Aly (uniprotkb:Q86V81) physically interacts (MI:0915) with ORF57 (uniprotkb:Q2HR75) by pull down (MI:0096). MINT-7291330, MINT-7291320: Nucleolin (uniprotkb:P19338) and ORF57 (uniprotkb:Q2HR75) colocalise (MI:0403) by fluorescence microscopy (MI:0416). MINT-7291715: ORF57 (uniprotkb:Q2HR75) and Aly (uniprotkb:Q86V81) colocalise (MI:0403) by fluorescence microscopy (MI:0416).",

keywords = "Herpesvirus, mRNA, Nuclear export, Nucleolus",

author = "Boyne, {James R.} and Adrian Whitehouse",

year = "2009",

month = nov,

day = "19",

doi = "10.1016/j.febslet.2009.10.040",

language = "English",

volume = "583",

pages = "3549--3556",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "22",

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T1 - Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs

AU - Boyne, James R.

AU - Whitehouse, Adrian

PY - 2009/11/19

Y1 - 2009/11/19

N2 - Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, the ability of ORF57 to export mRNA was assessed in cells where the nucleolus was disrupted using Actinomycin D or DRB. We show that rapid disorganisation of the nucleolus was accompanied by a reduction in intronless virus mRNA export, suggesting that an intact nucleolus is essential for efficient KSHV ORF57-mediated intronless mRNA export. Structured summary: MINT-7291377: Aly (uniprotkb:Q86V81) physically interacts (MI:0915) with ORF57 (uniprotkb:Q2HR75) by pull down (MI:0096). MINT-7291330, MINT-7291320: Nucleolin (uniprotkb:P19338) and ORF57 (uniprotkb:Q2HR75) colocalise (MI:0403) by fluorescence microscopy (MI:0416). MINT-7291715: ORF57 (uniprotkb:Q2HR75) and Aly (uniprotkb:Q86V81) colocalise (MI:0403) by fluorescence microscopy (MI:0416).

AB - Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, the ability of ORF57 to export mRNA was assessed in cells where the nucleolus was disrupted using Actinomycin D or DRB. We show that rapid disorganisation of the nucleolus was accompanied by a reduction in intronless virus mRNA export, suggesting that an intact nucleolus is essential for efficient KSHV ORF57-mediated intronless mRNA export. Structured summary: MINT-7291377: Aly (uniprotkb:Q86V81) physically interacts (MI:0915) with ORF57 (uniprotkb:Q2HR75) by pull down (MI:0096). MINT-7291330, MINT-7291320: Nucleolin (uniprotkb:P19338) and ORF57 (uniprotkb:Q2HR75) colocalise (MI:0403) by fluorescence microscopy (MI:0416). MINT-7291715: ORF57 (uniprotkb:Q2HR75) and Aly (uniprotkb:Q86V81) colocalise (MI:0403) by fluorescence microscopy (MI:0416).

KW - Herpesvirus

KW - mRNA

KW - Nuclear export

KW - Nucleolus

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DO - 10.1016/j.febslet.2009.10.040

M3 - Article

C2 - 19850040

AN - SCOPUS:71749109176

VL - 583

SP - 3549

EP - 3556

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 22

ER -

Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs

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