NuMA overexpression in epithelial ovarian cancer

Anke Brüning-Richardson, Jaqueline Bond, Rawiah Alsiary, Julie Richardson, David A. Cairns, Luci McCormac, Richard Hutson, Philip A. Burns, Nafisa Wilkinson, Geoff D. Hall, Ewan E. Morrison, Sandra M. Bell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Highly aneuploid tumours are common in epithelial ovarian cancers (EOC). We investigated whether NuMA expression was associated with this phenomenon. NuMA protein levels in normal and tumour tissues, ovarian cell lines and primary cultures of malignant cells derived from ovarian ascitic fluids were analysed by Affymetrix microarray analysis, immunoblotting, immunohistochemistry (IHC) and immunofluorescence (IF), with results correlated to associated clinical data. Aneuploidy status in primary cultures was determined by FACS analysis. Affymetrix microarray data indicated that NuMA was overexpressed in tumour tissue, primary cultures and cell lines compared to normal ovarian tissue. IHC revealed low to weak NuMA expression in normal tissues. Expression was upregulated in tumours, with a significant association with disease stage in mucinous EOC subtypes (p = 0.009), lymph node involvement (p = 0.03) and patient age (p = 0.04). Additional discontinuous data analysis revealed that high NuMA levels in tumours decreased with grade (p = 0.02) but increased with disease stage (p = 0.04) in serous EOC. NuMA expression decreased in late disease stage 4 endometrioid EOCs. High NuMA levels decreased with increased tumour invasion in all subtypes (p = 0.03). IF of primary cultures revealed that high NuMA levels at mitotic spindle poles were significantly associated with a decreased proportion of cells in cytokinesis (p = 0.05), increased binucleation (p = 0.021) and multinucleation (p = 0.007), and aneuploidy (p = 0.008). NuMA is highly expressed in EOC tumours and high NuMA levels correlate with increases in mitotic defects and aneuploidy in primary cultures.

Original languageEnglish
Article numbere38945
JournalPLoS One
Volume7
Issue number6
DOIs
Publication statusPublished - 14 Jun 2012
Externally publishedYes

Fingerprint

ovarian neoplasms
Tumors
aneuploidy
Aneuploidy
neoplasms
Neoplasms
Tissue
Microarrays
Cell culture
fluorescent antibody technique
Fluorescent Antibody Technique
immunohistochemistry
Immunohistochemistry
Cells
cell lines
Spindle Poles
Cell Line
mitotic spindle apparatus
Spindle Apparatus
Primary Cell Culture

Cite this

Brüning-Richardson, A., Bond, J., Alsiary, R., Richardson, J., Cairns, D. A., McCormac, L., ... Bell, S. M. (2012). NuMA overexpression in epithelial ovarian cancer. PLoS One, 7(6), [e38945]. https://doi.org/10.1371/journal.pone.0038945
Brüning-Richardson, Anke ; Bond, Jaqueline ; Alsiary, Rawiah ; Richardson, Julie ; Cairns, David A. ; McCormac, Luci ; Hutson, Richard ; Burns, Philip A. ; Wilkinson, Nafisa ; Hall, Geoff D. ; Morrison, Ewan E. ; Bell, Sandra M. / NuMA overexpression in epithelial ovarian cancer. In: PLoS One. 2012 ; Vol. 7, No. 6.
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Brüning-Richardson, A, Bond, J, Alsiary, R, Richardson, J, Cairns, DA, McCormac, L, Hutson, R, Burns, PA, Wilkinson, N, Hall, GD, Morrison, EE & Bell, SM 2012, 'NuMA overexpression in epithelial ovarian cancer', PLoS One, vol. 7, no. 6, e38945. https://doi.org/10.1371/journal.pone.0038945

NuMA overexpression in epithelial ovarian cancer. / Brüning-Richardson, Anke; Bond, Jaqueline; Alsiary, Rawiah; Richardson, Julie; Cairns, David A.; McCormac, Luci; Hutson, Richard; Burns, Philip A.; Wilkinson, Nafisa; Hall, Geoff D.; Morrison, Ewan E.; Bell, Sandra M.

In: PLoS One, Vol. 7, No. 6, e38945, 14.06.2012.

Research output: Contribution to journalArticle

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AU - Brüning-Richardson, Anke

AU - Bond, Jaqueline

AU - Alsiary, Rawiah

AU - Richardson, Julie

AU - Cairns, David A.

AU - McCormac, Luci

AU - Hutson, Richard

AU - Burns, Philip A.

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AU - Hall, Geoff D.

AU - Morrison, Ewan E.

AU - Bell, Sandra M.

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Brüning-Richardson A, Bond J, Alsiary R, Richardson J, Cairns DA, McCormac L et al. NuMA overexpression in epithelial ovarian cancer. PLoS One. 2012 Jun 14;7(6). e38945. https://doi.org/10.1371/journal.pone.0038945