On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: A short review

Gordon A. Morris, Gary G. Adams, Stephen E. Harding

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38 Citations (Scopus)


Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance. Discussion: Here the use of hydrodynamic tools such as sedimentation velocity, sedimentation equilibrium, size exclusion chromatography - multi-angle light scattering (SEC-MALS), and viscometry are considered highlighting some recent developments in methodology and the application of these to help better understand polysaccharide structure-function relationships. Conclusions: The size and shape of polysaccharides in solution can be estimated in a variety of ways. Molar masses and heterogeneities can be estimated to a good precision by sedimentation velocity, sedimentation equilibrium and SEC-MALS. An approximate idea of conformation and flexibility can be obtained from power-law coefficients and the Wales van Holde parameter. More sophisticated estimates can be obtained by combining methods together to yield the persistence length.
Original languageEnglish
Pages (from-to)318-334
JournalFood Hydrocolloids
Issue numberP3
Publication statusPublished - 5 Dec 2014


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