On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: A short review

Gordon A. Morris, Gary G. Adams, Stephen E. Harding

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Polysaccharides and their derivatives are increasingly being used by the food, cosmetic and pharmaceutical industries: physical properties like size and conformation are important contributors to their performance. Discussion: Here the use of hydrodynamic tools such as sedimentation velocity, sedimentation equilibrium, size exclusion chromatography - multi-angle light scattering (SEC-MALS), and viscometry are considered highlighting some recent developments in methodology and the application of these to help better understand polysaccharide structure-function relationships. Conclusions: The size and shape of polysaccharides in solution can be estimated in a variety of ways. Molar masses and heterogeneities can be estimated to a good precision by sedimentation velocity, sedimentation equilibrium and SEC-MALS. An approximate idea of conformation and flexibility can be obtained from power-law coefficients and the Wales van Holde parameter. More sophisticated estimates can be obtained by combining methods together to yield the persistence length.
LanguageEnglish
Pages318-334
JournalFood Hydrocolloids
Volume42
Issue numberP3
DOIs
Publication statusPublished - 5 Dec 2014

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Hydrodynamics
Polysaccharides
Sedimentation
hydrodynamics
polysaccharides
light scattering
Gel Chromatography
Size exclusion chromatography
Light scattering
viscometry
Conformations
Light
Food Industry
Wales
Drug Industry
structure-activity relationships
cosmetics
Cosmetics
Molar mass
physical properties

Cite this

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On hydrodynamic methods for the analysis of the sizes and shapes of polysaccharides in dilute solution: A short review. / Morris, Gordon A.; Adams, Gary G.; Harding, Stephen E.

In: Food Hydrocolloids, Vol. 42, No. P3, 05.12.2014, p. 318-334.

Research output: Contribution to journalArticle

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AU - Adams, Gary G.

AU - Harding, Stephen E.

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