Optimisation of Fungal Cellulase Production from Textile Waste Using Experimental Design

Yunzi Hu, Chenyu Du, Nattha Pensupa, Carol Sze Ki Lin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Abundant textile waste has raised increasing concerns worldwide in developing novel circular textiles approach. This study investigated the optimum cellulase production from textile waste by Aspergillus niger CKB. Textile wastes consisting of cotton and polyester in various ratios were used as low-cost feedstock. Three types of cultivation media were compared in solid state fermentation and Mandels medium with yeast extract selected due to its superior cellulase production. Conditions including moisture, pH, inoculum size and organic nitrogen were evaluated and optimised via response surface methodology. Supplementary carbon sources and cellulase inducers were also employed to enhance fungal growth and cellulase production. The results indicated that the optimised fermentation method significantly improved cellulase producing efficiency and enzyme activity by 88.7% and 25.8%, respectively. The maximum cellulase activity reached 1.56 FPU g−1 in 6 days. The outcomes led to the efficient recovery of glucose and polyester, which could contribute to a closed-loop recycling strategy for the textile industry, and enable the transition to an advanced circular textiles economy.

LanguageEnglish
Pages133-142
Number of pages10
JournalProcess Safety and Environmental Protection
Volume118
Early online date27 Jun 2018
DOIs
Publication statusPublished - 1 Aug 2018

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Cellulase
experimental design
Design of experiments
Textiles
Polyesters
Fermentation
fermentation
Aspergillus
Enzyme activity
textile industry
Textile industry
organic nitrogen
Yeast
Feedstocks
Cotton
enzyme activity
yeast
Glucose
Recycling
cotton

Cite this

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title = "Optimisation of Fungal Cellulase Production from Textile Waste Using Experimental Design",
abstract = "Abundant textile waste has raised increasing concerns worldwide in developing novel circular textiles approach. This study investigated the optimum cellulase production from textile waste by Aspergillus niger CKB. Textile wastes consisting of cotton and polyester in various ratios were used as low-cost feedstock. Three types of cultivation media were compared in solid state fermentation and Mandels medium with yeast extract selected due to its superior cellulase production. Conditions including moisture, pH, inoculum size and organic nitrogen were evaluated and optimised via response surface methodology. Supplementary carbon sources and cellulase inducers were also employed to enhance fungal growth and cellulase production. The results indicated that the optimised fermentation method significantly improved cellulase producing efficiency and enzyme activity by 88.7{\%} and 25.8{\%}, respectively. The maximum cellulase activity reached 1.56 FPU g−1 in 6 days. The outcomes led to the efficient recovery of glucose and polyester, which could contribute to a closed-loop recycling strategy for the textile industry, and enable the transition to an advanced circular textiles economy.",
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Optimisation of Fungal Cellulase Production from Textile Waste Using Experimental Design. / Hu, Yunzi; Du, Chenyu; Pensupa, Nattha; Lin, Carol Sze Ki.

In: Process Safety and Environmental Protection, Vol. 118, 01.08.2018, p. 133-142.

Research output: Contribution to journalArticle

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T1 - Optimisation of Fungal Cellulase Production from Textile Waste Using Experimental Design

AU - Hu, Yunzi

AU - Du, Chenyu

AU - Pensupa, Nattha

AU - Lin, Carol Sze Ki

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Y1 - 2018/8/1

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AB - Abundant textile waste has raised increasing concerns worldwide in developing novel circular textiles approach. This study investigated the optimum cellulase production from textile waste by Aspergillus niger CKB. Textile wastes consisting of cotton and polyester in various ratios were used as low-cost feedstock. Three types of cultivation media were compared in solid state fermentation and Mandels medium with yeast extract selected due to its superior cellulase production. Conditions including moisture, pH, inoculum size and organic nitrogen were evaluated and optimised via response surface methodology. Supplementary carbon sources and cellulase inducers were also employed to enhance fungal growth and cellulase production. The results indicated that the optimised fermentation method significantly improved cellulase producing efficiency and enzyme activity by 88.7% and 25.8%, respectively. The maximum cellulase activity reached 1.56 FPU g−1 in 6 days. The outcomes led to the efficient recovery of glucose and polyester, which could contribute to a closed-loop recycling strategy for the textile industry, and enable the transition to an advanced circular textiles economy.

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