Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Jwan J. Abdullah, Darren Greetham, Nattha Pensupa, Gregory A. Tucker, Chenyu Du

Research output: Contribution to journalArticlepeer-review


This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the MSW substrate and production of cellulase enzymes was optimized for temperature, moisture content, inoculation and period of incubation. The effect of additional minerals, and alternative carbon and nitrogen sources were also examined.

Following optimization a cellulase activity of 26.10 ± 3.09 FPU/g could be produced using T. reesei at 30°C with a moisture content of 60% with an inoculums of 0.5 million spores/g and incubation for 168 hours. Addition of extra nitrogen and/or carbon did not improve cellulase accumulation. Acid or alkali pretreatment of MSW led to reduced cellulase production. Crude enzymes produced from MSW by T. reesei were evaluated for their ability to release glucose from MSW. A cellulose hydrolysis yield of 24.7% was achieved, which was close to that obtained using a commercial enzyme. Results demonstrated that MSW can be used as an inexpensive lignocellulosic material for the production of cellulase enzymes
Original languageEnglish
Article number10000206
Number of pages10
JournalJournal of Fundamentals of Renewable Energy and Applications
Issue number3
Publication statusPublished - 6 Apr 2016


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