Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni

Waheed Jowiya, Katja Brunner, Sherif Abouelhadid, Haitham A. Hussain, Sean P. Nair, Sohaib Sadiq, Lisa K. Williams, Emma K. Trantham, Holly Stephenson, Brendan W. Wren, Mona Bajaj-Elliott, Tristan A. Cogan, Andrew P. Laws, Jim Wade, Nick Dorrell, Elaine Allan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro, suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.

Original languageEnglish
Pages (from-to)4884-4895
Number of pages12
JournalInfection and Immunity
Volume83
Issue number12
DOIs
Publication statusPublished - 2015

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Campylobacter jejuni
Amylases
Biofilms
Dextrans
Gastroenteritis
Ileum
Intestines
Birds
Virulence
Chickens
Peptide Hydrolases
Epithelial Cells
Bacteria
Cell Line
Food
Infection
In Vitro Techniques

Cite this

Jowiya, Waheed ; Brunner, Katja ; Abouelhadid, Sherif ; Hussain, Haitham A. ; Nair, Sean P. ; Sadiq, Sohaib ; Williams, Lisa K. ; Trantham, Emma K. ; Stephenson, Holly ; Wren, Brendan W. ; Bajaj-Elliott, Mona ; Cogan, Tristan A. ; Laws, Andrew P. ; Wade, Jim ; Dorrell, Nick ; Allan, Elaine. / Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni. In: Infection and Immunity. 2015 ; Vol. 83, No. 12. pp. 4884-4895.
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abstract = "Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro, suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.",
author = "Waheed Jowiya and Katja Brunner and Sherif Abouelhadid and Hussain, {Haitham A.} and Nair, {Sean P.} and Sohaib Sadiq and Williams, {Lisa K.} and Trantham, {Emma K.} and Holly Stephenson and Wren, {Brendan W.} and Mona Bajaj-Elliott and Cogan, {Tristan A.} and Laws, {Andrew P.} and Jim Wade and Nick Dorrell and Elaine Allan",
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Jowiya, W, Brunner, K, Abouelhadid, S, Hussain, HA, Nair, SP, Sadiq, S, Williams, LK, Trantham, EK, Stephenson, H, Wren, BW, Bajaj-Elliott, M, Cogan, TA, Laws, AP, Wade, J, Dorrell, N & Allan, E 2015, 'Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni', Infection and Immunity, vol. 83, no. 12, pp. 4884-4895. https://doi.org/10.1128/IAI.01064-15

Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni. / Jowiya, Waheed; Brunner, Katja; Abouelhadid, Sherif; Hussain, Haitham A.; Nair, Sean P.; Sadiq, Sohaib; Williams, Lisa K.; Trantham, Emma K.; Stephenson, Holly; Wren, Brendan W.; Bajaj-Elliott, Mona; Cogan, Tristan A.; Laws, Andrew P.; Wade, Jim; Dorrell, Nick; Allan, Elaine.

In: Infection and Immunity, Vol. 83, No. 12, 2015, p. 4884-4895.

Research output: Contribution to journalArticle

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T1 - Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni

AU - Jowiya, Waheed

AU - Brunner, Katja

AU - Abouelhadid, Sherif

AU - Hussain, Haitham A.

AU - Nair, Sean P.

AU - Sadiq, Sohaib

AU - Williams, Lisa K.

AU - Trantham, Emma K.

AU - Stephenson, Holly

AU - Wren, Brendan W.

AU - Bajaj-Elliott, Mona

AU - Cogan, Tristan A.

AU - Laws, Andrew P.

AU - Wade, Jim

AU - Dorrell, Nick

AU - Allan, Elaine

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