Penetration of chlorhexidine into human skin

T. J. Karpanen, T. Worthington, B. R. Conway, A. C. Hilton, T. S J Elliott, P. A. Lambert

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

This study evaluated a model of skin permeation to determine the depth of delivery of chlorhexidine into full-thickness excised human skin following topical application of 2% (wt/vol) aqueous chlorhexidine digluconate. Skin permeation studies were performed on full-thickness human skin using Franz diffusion cells with exposure to chlorhexidine for 2 min, 30 min, and 24 h. The concentration of chlorhexidine extracted from skin sections was determined to a depth of 1,500 μm following serial sectioning of the skin using a microtome and analysis by high-performance liquid chromatography. Poor penetration of chlorhexidine into skin following 2-min and 30-min exposures to chlorhexidine was observed (0.157 ± 0.047 and 0.077 ± 0.015 μg/mg tissue within the top 100 μm), and levels of chlorhexidine were minimal at deeper skin depths (less than 0.002 μg/mg tissue below 300 μm). After 24 h of exposure, there was more chlorhexidine within the upper 100-μm sections (7.88 ± 1.37 μg/mg tissue); however, the levels remained low (less than 1 μg/mg tissue) at depths below 300 μm. There was no detectable penetration through the full-thickness skin. The model presented in this study can be used to assess the permeation of antiseptic agents through various layers of skin in vitro. Aqueous chlorhexidine demonstrated poor permeation into the deeper layers of the skin, which may restrict the efficacy of skin antisepsis with this agent. This study lays the foundation for further research in adopting alternative strategies for enhanced skin antisepsis in clinical practice.

LanguageEnglish
Pages3633-3636
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume52
Issue number10
Early online date1 Aug 2008
DOIs
Publication statusPublished - 1 Oct 2008
Externally publishedYes

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Chlorhexidine
Skin
Antisepsis
Local Anti-Infective Agents

Cite this

Karpanen, T. J., Worthington, T., Conway, B. R., Hilton, A. C., Elliott, T. S. J., & Lambert, P. A. (2008). Penetration of chlorhexidine into human skin. Antimicrobial Agents and Chemotherapy, 52(10), 3633-3636. https://doi.org/10.1128/AAC.00637-08
Karpanen, T. J. ; Worthington, T. ; Conway, B. R. ; Hilton, A. C. ; Elliott, T. S J ; Lambert, P. A. / Penetration of chlorhexidine into human skin. In: Antimicrobial Agents and Chemotherapy. 2008 ; Vol. 52, No. 10. pp. 3633-3636.
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Karpanen, TJ, Worthington, T, Conway, BR, Hilton, AC, Elliott, TSJ & Lambert, PA 2008, 'Penetration of chlorhexidine into human skin', Antimicrobial Agents and Chemotherapy, vol. 52, no. 10, pp. 3633-3636. https://doi.org/10.1128/AAC.00637-08

Penetration of chlorhexidine into human skin. / Karpanen, T. J.; Worthington, T.; Conway, B. R.; Hilton, A. C.; Elliott, T. S J; Lambert, P. A.

In: Antimicrobial Agents and Chemotherapy, Vol. 52, No. 10, 01.10.2008, p. 3633-3636.

Research output: Contribution to journalArticle

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Karpanen TJ, Worthington T, Conway BR, Hilton AC, Elliott TSJ, Lambert PA. Penetration of chlorhexidine into human skin. Antimicrobial Agents and Chemotherapy. 2008 Oct 1;52(10):3633-3636. https://doi.org/10.1128/AAC.00637-08