Abstract
A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 45-50 |
| Number of pages | 6 |
| Journal | Journal of Controlled Release |
| Volume | 185 |
| Issue number | 1 |
| Early online date | 13 Apr 2014 |
| DOIs | |
| Publication status | Published - 10 Jul 2014 |
| Externally published | Yes |
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Penetration of normal, damaged and diseased skin : An in vitro study on dendritic core-multishell nanotransporters. / Alnasif, Nesrin; Zoschke, Christian; Fleige, Emanuel; Brodwolf, Robert; Boreham, Alexander; Rühl, Eckart; Eckl, Katja Martina; Merk, Hans Friedrich; Hennies, Hans Christian; Alexiev, Ulrike; Haag, Rainer; Küchler, Sarah; Schäfer-Korting, Monika.
In: Journal of Controlled Release, Vol. 185, No. 1, 10.07.2014, p. 45-50.Research output: Contribution to journal › Article
TY - JOUR
T1 - Penetration of normal, damaged and diseased skin
T2 - An in vitro study on dendritic core-multishell nanotransporters
AU - Alnasif, Nesrin
AU - Zoschke, Christian
AU - Fleige, Emanuel
AU - Brodwolf, Robert
AU - Boreham, Alexander
AU - Rühl, Eckart
AU - Eckl, Katja Martina
AU - Merk, Hans Friedrich
AU - Hennies, Hans Christian
AU - Alexiev, Ulrike
AU - Haag, Rainer
AU - Küchler, Sarah
AU - Schäfer-Korting, Monika
N1 - No full text in Eprints. HN 17/11/2017
PY - 2014/7/10
Y1 - 2014/7/10
N2 - A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.
AB - A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.
KW - Dendritic core-multishell nanotransporters
KW - Nanotoxicology
KW - Non-melanoma skin cancer
KW - Peeling skin syndrome
KW - Reconstructed human skin
KW - Skin absorption
UR - http://www.scopus.com/inward/record.url?scp=84900411111&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2014.04.006
DO - 10.1016/j.jconrel.2014.04.006
M3 - Article
C2 - 24727058
AN - SCOPUS:84900411111
VL - 185
SP - 45
EP - 50
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 1
ER -