Penetration of normal, damaged and diseased skin

An in vitro study on dendritic core-multishell nanotransporters

Nesrin Alnasif, Christian Zoschke, Emanuel Fleige, Robert Brodwolf, Alexander Boreham, Eckart Rühl, Katja Martina Eckl, Hans Friedrich Merk, Hans Christian Hennies, Ulrike Alexiev, Rainer Haag, Sarah Küchler, Monika Schäfer-Korting

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalJournal of Controlled Release
Volume185
Issue number1
Early online date13 Apr 2014
DOIs
Publication statusPublished - 10 Jul 2014
Externally publishedYes

Fingerprint

Skin Diseases
Skin
Coloring Agents
In Vitro Techniques
Optical Imaging
Skin Neoplasms
Dermis
Fluorescence Microscopy
Epidermis
Nanoparticles
Cornea
Microscopy

Cite this

Alnasif, N., Zoschke, C., Fleige, E., Brodwolf, R., Boreham, A., Rühl, E., ... Schäfer-Korting, M. (2014). Penetration of normal, damaged and diseased skin: An in vitro study on dendritic core-multishell nanotransporters. Journal of Controlled Release, 185(1), 45-50. https://doi.org/10.1016/j.jconrel.2014.04.006
Alnasif, Nesrin ; Zoschke, Christian ; Fleige, Emanuel ; Brodwolf, Robert ; Boreham, Alexander ; Rühl, Eckart ; Eckl, Katja Martina ; Merk, Hans Friedrich ; Hennies, Hans Christian ; Alexiev, Ulrike ; Haag, Rainer ; Küchler, Sarah ; Schäfer-Korting, Monika. / Penetration of normal, damaged and diseased skin : An in vitro study on dendritic core-multishell nanotransporters. In: Journal of Controlled Release. 2014 ; Vol. 185, No. 1. pp. 45-50.
@article{aa6b02dc7ab24fd2b1b8489f681366b1,
title = "Penetration of normal, damaged and diseased skin: An in vitro study on dendritic core-multishell nanotransporters",
abstract = "A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.",
keywords = "Dendritic core-multishell nanotransporters, Nanotoxicology, Non-melanoma skin cancer, Peeling skin syndrome, Reconstructed human skin, Skin absorption",
author = "Nesrin Alnasif and Christian Zoschke and Emanuel Fleige and Robert Brodwolf and Alexander Boreham and Eckart R{\"u}hl and Eckl, {Katja Martina} and Merk, {Hans Friedrich} and Hennies, {Hans Christian} and Ulrike Alexiev and Rainer Haag and Sarah K{\"u}chler and Monika Sch{\"a}fer-Korting",
note = "No full text in Eprints. HN 17/11/2017",
year = "2014",
month = "7",
day = "10",
doi = "10.1016/j.jconrel.2014.04.006",
language = "English",
volume = "185",
pages = "45--50",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
number = "1",

}

Alnasif, N, Zoschke, C, Fleige, E, Brodwolf, R, Boreham, A, Rühl, E, Eckl, KM, Merk, HF, Hennies, HC, Alexiev, U, Haag, R, Küchler, S & Schäfer-Korting, M 2014, 'Penetration of normal, damaged and diseased skin: An in vitro study on dendritic core-multishell nanotransporters', Journal of Controlled Release, vol. 185, no. 1, pp. 45-50. https://doi.org/10.1016/j.jconrel.2014.04.006

Penetration of normal, damaged and diseased skin : An in vitro study on dendritic core-multishell nanotransporters. / Alnasif, Nesrin; Zoschke, Christian; Fleige, Emanuel; Brodwolf, Robert; Boreham, Alexander; Rühl, Eckart; Eckl, Katja Martina; Merk, Hans Friedrich; Hennies, Hans Christian; Alexiev, Ulrike; Haag, Rainer; Küchler, Sarah; Schäfer-Korting, Monika.

In: Journal of Controlled Release, Vol. 185, No. 1, 10.07.2014, p. 45-50.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Penetration of normal, damaged and diseased skin

T2 - An in vitro study on dendritic core-multishell nanotransporters

AU - Alnasif, Nesrin

AU - Zoschke, Christian

AU - Fleige, Emanuel

AU - Brodwolf, Robert

AU - Boreham, Alexander

AU - Rühl, Eckart

AU - Eckl, Katja Martina

AU - Merk, Hans Friedrich

AU - Hennies, Hans Christian

AU - Alexiev, Ulrike

AU - Haag, Rainer

AU - Küchler, Sarah

AU - Schäfer-Korting, Monika

N1 - No full text in Eprints. HN 17/11/2017

PY - 2014/7/10

Y1 - 2014/7/10

N2 - A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.

AB - A growing intended or accidental exposure to nanoparticles asks for the elucidation of potential toxicity linked to the penetration of normal and lesional skin. We studied the skin penetration of dye-tagged dendritic core-multishell (CMS) nanotransporters and of Nile red loaded CMS nanotransporters using fluorescence microscopy. Normal and stripped human skin ex vivo as well as normal reconstructed human skin and in vitro skin disease models served as test platforms. Nile red was delivered rapidly into the viable epidermis and dermis of normal skin, whereas the highly flexible CMS nanotransporters remained solely in the stratum corneum after 6 h but penetrated into deeper skin layers after 24 h exposure. Fluorescence lifetime imaging microscopy proved a stable dye-tag and revealed striking nanotransporter-skin interactions. The viable layers of stripped skin were penetrated more efficiently by dye-tagged CMS nanotransporters and the cargo compared to normal skin. Normal reconstructed human skin reflected the penetration of Nile red and CMS nanotransporters in human skin and both, the non-hyperkeratotic non-melanoma skin cancer and hyperkeratotic peeling skin disease models come along with altered absorption in the skin diseases.

KW - Dendritic core-multishell nanotransporters

KW - Nanotoxicology

KW - Non-melanoma skin cancer

KW - Peeling skin syndrome

KW - Reconstructed human skin

KW - Skin absorption

UR - http://www.scopus.com/inward/record.url?scp=84900411111&partnerID=8YFLogxK

U2 - 10.1016/j.jconrel.2014.04.006

DO - 10.1016/j.jconrel.2014.04.006

M3 - Article

VL - 185

SP - 45

EP - 50

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 1

ER -