Peptidase activity of β-lactamases

Noureddine Rhazi, Moreno Galleni, Michael I. Page, Jean Marie Frère

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Although β-lactamases have generally been considered as being devoid of peptidase activity, a low but significant hydrolysis of various N-acylated dipeptides was observed with representatives of each class of β-lactamases. The k(cat)/K(m) values were below 0.1 M-1.s-1, but the enzyme rate enhancement factors were in the range 5000-20000 for the best substrates. Not unexpectedly, the best 'peptidase' was the class C β-lactamase of Enterobacter cloacae P99, but, more surprisingly, the activity was always higher with the phenylacetyl- and benzoyl-D-Ala-D-Ala dipeptides than with the diacetyl- and α-acetyl-L-Lys-D-Ala-D-Ala tripeptides, which are the preferred substrates of the low-molecular-mass, soluble DD-peptidases. A comparison between the β-lactamases and DD-peptidases showed that it might be as difficult for a DD-peptidase to open the β-lactam ring as it is for the β-lacetamases to hydrolyse the peptides, an observation which can be explained by geometric and stereoelectronic considerations.

LanguageEnglish
Pages409-413
Number of pages5
JournalBiochemical Journal
Volume341
Issue number2
DOIs
Publication statusPublished - 15 Jul 1999

Fingerprint

Serine-Type D-Ala-D-Ala Carboxypeptidase
beta-Lactamases
Peptide Hydrolases
Dipeptides
Diacetyl
Enterobacter cloacae
Molecular mass
beta-Lactams
Substrates
Hydrolysis
Observation
Peptides
Enzymes
alanylalanine

Cite this

Rhazi, N., Galleni, M., Page, M. I., & Frère, J. M. (1999). Peptidase activity of β-lactamases. Biochemical Journal, 341(2), 409-413. https://doi.org/10.1042/0264-6021:3410409
Rhazi, Noureddine ; Galleni, Moreno ; Page, Michael I. ; Frère, Jean Marie. / Peptidase activity of β-lactamases. In: Biochemical Journal. 1999 ; Vol. 341, No. 2. pp. 409-413.
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Rhazi, N, Galleni, M, Page, MI & Frère, JM 1999, 'Peptidase activity of β-lactamases', Biochemical Journal, vol. 341, no. 2, pp. 409-413. https://doi.org/10.1042/0264-6021:3410409

Peptidase activity of β-lactamases. / Rhazi, Noureddine; Galleni, Moreno; Page, Michael I.; Frère, Jean Marie.

In: Biochemical Journal, Vol. 341, No. 2, 15.07.1999, p. 409-413.

Research output: Contribution to journalArticle

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Rhazi N, Galleni M, Page MI, Frère JM. Peptidase activity of β-lactamases. Biochemical Journal. 1999 Jul 15;341(2):409-413. https://doi.org/10.1042/0264-6021:3410409