Peptide Biomarkers for Identifying the Species Origin of Gelatin Using Coupled UPLC-MS/MS

Sean Ward, Nicholas Powles, Michael I. Page

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Liquid chromatography linked with mass spectrometry (LC–MS/MS) was used to analyse gelatin from four different species after a trypsin digest. Using chemometric software to analyse the data it was possible to find peptide fragments that were specific to each species of gelatin: porcine, bovine, chicken or fish. Identification of these peptides was challenging due to the destructive nature of gelatin manufacture. The untargeted workflow method developed allowed identification of 21 unknown gelatin samples with 100% accuracy. Fish gelatin is made from a large range of different species that do not share a common differentiating protein but it was shown that the protein from a parasitic bacteria could be used to identify fish gelatin.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalJournal of Food Composition and Analysis
Volume73
Early online date18 Aug 2018
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Gelatin
gelatin
biomarkers
Biomarkers
peptides
Peptides
Fishes
fish
Peptide Fragments
Workflow
chemometrics
Liquid Chromatography
Trypsin
trypsin
liquid chromatography
tandem mass spectrometry
Chickens
Mass Spectrometry
manufacturing
Proteins

Cite this

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abstract = "Liquid chromatography linked with mass spectrometry (LC–MS/MS) was used to analyse gelatin from four different species after a trypsin digest. Using chemometric software to analyse the data it was possible to find peptide fragments that were specific to each species of gelatin: porcine, bovine, chicken or fish. Identification of these peptides was challenging due to the destructive nature of gelatin manufacture. The untargeted workflow method developed allowed identification of 21 unknown gelatin samples with 100{\%} accuracy. Fish gelatin is made from a large range of different species that do not share a common differentiating protein but it was shown that the protein from a parasitic bacteria could be used to identify fish gelatin.",
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Peptide Biomarkers for Identifying the Species Origin of Gelatin Using Coupled UPLC-MS/MS. / Ward, Sean; Powles, Nicholas; Page, Michael I.

In: Journal of Food Composition and Analysis, Vol. 73, 01.10.2018, p. 83-90.

Research output: Contribution to journalArticle

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