Period and timeless mRNA Splicing Profiles under Natural Conditions in Drosophila melanogaster

Stefano Montelli, Gabriella Mazzotta, Stefano Vanin, Laura Caccin, Samantha Corrà, Cristiano De Pittà, Catharine Boothroyd, Edward W. Green, Charalambos P. Kyriacou, Rodolfo Costa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previous analysis of Drosophila circadian behavior under natural conditions has revealed a number of novel and unexpected features. Here we focus on the oscillations of per and tim mRNAs and their posttranscriptional regulation and observe significant differences in molecular cycling under laboratory and natural conditions. In particular, robust per mRNA cycling from fly heads is limited to the summers, whereas tim RNA cycling is observed throughout the year. When both transcripts do cycle, their phases are similar, except for the very warmest summer months. We also study the natural splicing profiles of per and tim transcripts and observe a clear relationship between temperature and splicing. In natural conditions, we confirm the relationship between accumulation of the perspliced variant, low temperature, and the onset of the evening component of locomotor activity, first described in laboratory conditions. Intriguingly, in the case of tim splicing, we detect the opposite relationship, with timspliced expression increasing at higher temperatures. A first characterization of the 4 different TIM protein isoforms (resulting from the combination of the natural N-terminus length polymorphism and the C-terminus alternative splicing) using the 2-hybrid assay showed that the TIMunspliced isoforms have a stronger affinity for CRY, but not for PER, suggesting that the tim 3′ splicing could have physiological significance, possibly in temperature entrainment and/or adaptation to seasonal environments.

LanguageEnglish
Pages217-227
Number of pages11
JournalJournal of Biological Rhythms
Volume30
Issue number3
DOIs
Publication statusPublished - 4 Jun 2015

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Drosophila melanogaster
Messenger RNA
Temperature
Protein Isoforms
Alternative Splicing
Locomotion
Diptera
Drosophila
Head
RNA

Cite this

Montelli, S., Mazzotta, G., Vanin, S., Caccin, L., Corrà, S., De Pittà, C., ... Costa, R. (2015). Period and timeless mRNA Splicing Profiles under Natural Conditions in Drosophila melanogaster. Journal of Biological Rhythms, 30(3), 217-227. https://doi.org/10.1177/0748730415583575
Montelli, Stefano ; Mazzotta, Gabriella ; Vanin, Stefano ; Caccin, Laura ; Corrà, Samantha ; De Pittà, Cristiano ; Boothroyd, Catharine ; Green, Edward W. ; Kyriacou, Charalambos P. ; Costa, Rodolfo. / Period and timeless mRNA Splicing Profiles under Natural Conditions in Drosophila melanogaster. In: Journal of Biological Rhythms. 2015 ; Vol. 30, No. 3. pp. 217-227.
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Montelli, S, Mazzotta, G, Vanin, S, Caccin, L, Corrà, S, De Pittà, C, Boothroyd, C, Green, EW, Kyriacou, CP & Costa, R 2015, 'Period and timeless mRNA Splicing Profiles under Natural Conditions in Drosophila melanogaster', Journal of Biological Rhythms, vol. 30, no. 3, pp. 217-227. https://doi.org/10.1177/0748730415583575

Period and timeless mRNA Splicing Profiles under Natural Conditions in Drosophila melanogaster. / Montelli, Stefano; Mazzotta, Gabriella; Vanin, Stefano; Caccin, Laura; Corrà, Samantha; De Pittà, Cristiano; Boothroyd, Catharine; Green, Edward W.; Kyriacou, Charalambos P.; Costa, Rodolfo.

In: Journal of Biological Rhythms, Vol. 30, No. 3, 04.06.2015, p. 217-227.

Research output: Contribution to journalArticle

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