Phenotype microarray technology and its application in industrial biotechnology

Research output: Contribution to journalReview article

9 Citations (Scopus)

Abstract

Phenotype microarray (PM) technology provides an insight into the metabolic profiling of microbial cells within 96-well plate system. The PM assay allows for cells to be assessed for utilisation of nutrients or sensitivity to toxic compounds. The assay utilises a redox sensitive tetrazolium dye which becomes irreversibly reduced upon detection of cellular metabolic output, detection is synchronous with a colour change from colourless to purple. Output from PM technology can be measured visually or quantified by reader the absorbance in each well. PM technology has highlighted differences in growth requirements, nutrient utilisation, sensitivity to toxins, and genetic diversity in bacteria, fungi and mammalian cells.

Original languageEnglish
Pages (from-to)1153-1160
Number of pages8
JournalBiotechnology Letters
Volume36
Issue number6
Early online date22 Feb 2014
DOIs
Publication statusPublished - 1 Jun 2014
Externally publishedYes

Fingerprint

Biotechnology
Microarrays
Technology
Phenotype
Nutrients
Assays
Food
Poisons
Fungi
Oxidation-Reduction
Bacteria
Coloring Agents
Dyes
Color
Cells
Growth

Cite this

@article{c34465a687ed4c3aa6fa77ed8649b306,
title = "Phenotype microarray technology and its application in industrial biotechnology",
abstract = "Phenotype microarray (PM) technology provides an insight into the metabolic profiling of microbial cells within 96-well plate system. The PM assay allows for cells to be assessed for utilisation of nutrients or sensitivity to toxic compounds. The assay utilises a redox sensitive tetrazolium dye which becomes irreversibly reduced upon detection of cellular metabolic output, detection is synchronous with a colour change from colourless to purple. Output from PM technology can be measured visually or quantified by reader the absorbance in each well. PM technology has highlighted differences in growth requirements, nutrient utilisation, sensitivity to toxins, and genetic diversity in bacteria, fungi and mammalian cells.",
keywords = "Live cells, Metabolic output, Metabolic profiling, Microarray technology, Phenotypic microarray",
author = "Darren Greetham",
year = "2014",
month = "6",
day = "1",
doi = "10.1007/s10529-014-1481-x",
language = "English",
volume = "36",
pages = "1153--1160",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "6",

}

Phenotype microarray technology and its application in industrial biotechnology. / Greetham, Darren.

In: Biotechnology Letters, Vol. 36, No. 6, 01.06.2014, p. 1153-1160.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Phenotype microarray technology and its application in industrial biotechnology

AU - Greetham, Darren

PY - 2014/6/1

Y1 - 2014/6/1

N2 - Phenotype microarray (PM) technology provides an insight into the metabolic profiling of microbial cells within 96-well plate system. The PM assay allows for cells to be assessed for utilisation of nutrients or sensitivity to toxic compounds. The assay utilises a redox sensitive tetrazolium dye which becomes irreversibly reduced upon detection of cellular metabolic output, detection is synchronous with a colour change from colourless to purple. Output from PM technology can be measured visually or quantified by reader the absorbance in each well. PM technology has highlighted differences in growth requirements, nutrient utilisation, sensitivity to toxins, and genetic diversity in bacteria, fungi and mammalian cells.

AB - Phenotype microarray (PM) technology provides an insight into the metabolic profiling of microbial cells within 96-well plate system. The PM assay allows for cells to be assessed for utilisation of nutrients or sensitivity to toxic compounds. The assay utilises a redox sensitive tetrazolium dye which becomes irreversibly reduced upon detection of cellular metabolic output, detection is synchronous with a colour change from colourless to purple. Output from PM technology can be measured visually or quantified by reader the absorbance in each well. PM technology has highlighted differences in growth requirements, nutrient utilisation, sensitivity to toxins, and genetic diversity in bacteria, fungi and mammalian cells.

KW - Live cells

KW - Metabolic output

KW - Metabolic profiling

KW - Microarray technology

KW - Phenotypic microarray

UR - http://www.scopus.com/inward/record.url?scp=84899965970&partnerID=8YFLogxK

U2 - 10.1007/s10529-014-1481-x

DO - 10.1007/s10529-014-1481-x

M3 - Review article

C2 - 24563312

AN - SCOPUS:84899965970

VL - 36

SP - 1153

EP - 1160

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 6

ER -