Phenotypic characterisation of Saccharomyces spp. for tolerance to 1-butanol

A. M. Zaki, T. T. Wimalasena, D. Greetham

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Biofuels are expected to play a role in replacing crude oil as a liquid transportation fuel, and research into butanol has highlighted the importance of this alcohol as a fuel. Butanol has a higher energy density than ethanol, butanol–gasoline blends do not separate in the presence of water, and butanol is miscible with gasoline (Szulczyk, Int J Energy Environ 1(1):2876–2895, 40). Saccharomyces cerevisiae has been used as a fermentative organism in the biofuel industry producing ethanol from glucose derived from starchy plant material; however, it typically cannot tolerate butanol concentrations greater than 2 % (Luong, Biotechnol Bioeng 29 (2):242–248, 27). 90 Saccharomyces spp. strains were screened for tolerance to 1-butanol via a phenotypic microarray assay and we observed significant variation in response with the most tolerant strains (S. cerevisiae DBVPG1788, S. cerevisiae DBVPG6044 and S. cerevisiae YPS128) exhibiting tolerance to 4 % 1-butanol compared with S. uvarum and S. castelli strains, which were sensitive to 3 % 1-butanol. Response to butanol was confirmed using traditional yeast methodologies such as growth; it was observed that fermentations in the presence of butanol, when using strains with a tolerant background, were significantly faster. Assessing for genetic rationale for tolerance, it was observed that 1-butanol-tolerant strains, when compared with 1-butanol-sensitive strains, had an up-regulation of RPN4, a transcription factor which regulates proteasome genes. Analysing for the importance of RPN4, we observed that a Δrpn4 strain displayed a reduced rate of fermentation in the presence of 1-butanol when compared with the BY4741 background strain. This data will aid the development of breeding programmes to produce better strains for future bio-butanol production.

LanguageEnglish
Pages1627-1636
Number of pages10
JournalJournal of Industrial Microbiology and Biotechnology
Volume41
Issue number11
Early online date23 Sep 2014
DOIs
Publication statusPublished - 1 Nov 2014
Externally publishedYes

Fingerprint

Butanols
Saccharomyces
1-Butanol
Butenes
Yeast
Saccharomyces cerevisiae
Gasoline
Biofuels
Fermentation
Ethanol
Program Development
Petroleum
Proteasome Endopeptidase Complex
Microarrays
Breeding
Industry
Transcription Factors
Up-Regulation
Yeasts
Assays

Cite this

@article{7cacd45d964a4f9fa06e50f7f79e45ff,
title = "Phenotypic characterisation of Saccharomyces spp. for tolerance to 1-butanol",
abstract = "Biofuels are expected to play a role in replacing crude oil as a liquid transportation fuel, and research into butanol has highlighted the importance of this alcohol as a fuel. Butanol has a higher energy density than ethanol, butanol–gasoline blends do not separate in the presence of water, and butanol is miscible with gasoline (Szulczyk, Int J Energy Environ 1(1):2876–2895, 40). Saccharomyces cerevisiae has been used as a fermentative organism in the biofuel industry producing ethanol from glucose derived from starchy plant material; however, it typically cannot tolerate butanol concentrations greater than 2 {\%} (Luong, Biotechnol Bioeng 29 (2):242–248, 27). 90 Saccharomyces spp. strains were screened for tolerance to 1-butanol via a phenotypic microarray assay and we observed significant variation in response with the most tolerant strains (S. cerevisiae DBVPG1788, S. cerevisiae DBVPG6044 and S. cerevisiae YPS128) exhibiting tolerance to 4 {\%} 1-butanol compared with S. uvarum and S. castelli strains, which were sensitive to 3 {\%} 1-butanol. Response to butanol was confirmed using traditional yeast methodologies such as growth; it was observed that fermentations in the presence of butanol, when using strains with a tolerant background, were significantly faster. Assessing for genetic rationale for tolerance, it was observed that 1-butanol-tolerant strains, when compared with 1-butanol-sensitive strains, had an up-regulation of RPN4, a transcription factor which regulates proteasome genes. Analysing for the importance of RPN4, we observed that a Δrpn4 strain displayed a reduced rate of fermentation in the presence of 1-butanol when compared with the BY4741 background strain. This data will aid the development of breeding programmes to produce better strains for future bio-butanol production.",
keywords = "1-Butanol, Fermentation, Phenotypic microarray, qPCR, Saccharomyces spp",
author = "Zaki, {A. M.} and Wimalasena, {T. T.} and D. Greetham",
year = "2014",
month = "11",
day = "1",
doi = "10.1007/s10295-014-1511-7",
language = "English",
volume = "41",
pages = "1627--1636",
journal = "Journal of Industrial Microbiology and Biotechnology",
issn = "1367-5435",
publisher = "Springer Verlag",
number = "11",

}

Phenotypic characterisation of Saccharomyces spp. for tolerance to 1-butanol. / Zaki, A. M.; Wimalasena, T. T.; Greetham, D.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 41, No. 11, 01.11.2014, p. 1627-1636.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phenotypic characterisation of Saccharomyces spp. for tolerance to 1-butanol

AU - Zaki, A. M.

AU - Wimalasena, T. T.

AU - Greetham, D.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Biofuels are expected to play a role in replacing crude oil as a liquid transportation fuel, and research into butanol has highlighted the importance of this alcohol as a fuel. Butanol has a higher energy density than ethanol, butanol–gasoline blends do not separate in the presence of water, and butanol is miscible with gasoline (Szulczyk, Int J Energy Environ 1(1):2876–2895, 40). Saccharomyces cerevisiae has been used as a fermentative organism in the biofuel industry producing ethanol from glucose derived from starchy plant material; however, it typically cannot tolerate butanol concentrations greater than 2 % (Luong, Biotechnol Bioeng 29 (2):242–248, 27). 90 Saccharomyces spp. strains were screened for tolerance to 1-butanol via a phenotypic microarray assay and we observed significant variation in response with the most tolerant strains (S. cerevisiae DBVPG1788, S. cerevisiae DBVPG6044 and S. cerevisiae YPS128) exhibiting tolerance to 4 % 1-butanol compared with S. uvarum and S. castelli strains, which were sensitive to 3 % 1-butanol. Response to butanol was confirmed using traditional yeast methodologies such as growth; it was observed that fermentations in the presence of butanol, when using strains with a tolerant background, were significantly faster. Assessing for genetic rationale for tolerance, it was observed that 1-butanol-tolerant strains, when compared with 1-butanol-sensitive strains, had an up-regulation of RPN4, a transcription factor which regulates proteasome genes. Analysing for the importance of RPN4, we observed that a Δrpn4 strain displayed a reduced rate of fermentation in the presence of 1-butanol when compared with the BY4741 background strain. This data will aid the development of breeding programmes to produce better strains for future bio-butanol production.

AB - Biofuels are expected to play a role in replacing crude oil as a liquid transportation fuel, and research into butanol has highlighted the importance of this alcohol as a fuel. Butanol has a higher energy density than ethanol, butanol–gasoline blends do not separate in the presence of water, and butanol is miscible with gasoline (Szulczyk, Int J Energy Environ 1(1):2876–2895, 40). Saccharomyces cerevisiae has been used as a fermentative organism in the biofuel industry producing ethanol from glucose derived from starchy plant material; however, it typically cannot tolerate butanol concentrations greater than 2 % (Luong, Biotechnol Bioeng 29 (2):242–248, 27). 90 Saccharomyces spp. strains were screened for tolerance to 1-butanol via a phenotypic microarray assay and we observed significant variation in response with the most tolerant strains (S. cerevisiae DBVPG1788, S. cerevisiae DBVPG6044 and S. cerevisiae YPS128) exhibiting tolerance to 4 % 1-butanol compared with S. uvarum and S. castelli strains, which were sensitive to 3 % 1-butanol. Response to butanol was confirmed using traditional yeast methodologies such as growth; it was observed that fermentations in the presence of butanol, when using strains with a tolerant background, were significantly faster. Assessing for genetic rationale for tolerance, it was observed that 1-butanol-tolerant strains, when compared with 1-butanol-sensitive strains, had an up-regulation of RPN4, a transcription factor which regulates proteasome genes. Analysing for the importance of RPN4, we observed that a Δrpn4 strain displayed a reduced rate of fermentation in the presence of 1-butanol when compared with the BY4741 background strain. This data will aid the development of breeding programmes to produce better strains for future bio-butanol production.

KW - 1-Butanol

KW - Fermentation

KW - Phenotypic microarray

KW - qPCR

KW - Saccharomyces spp

UR - http://www.scopus.com/inward/record.url?scp=84910140907&partnerID=8YFLogxK

U2 - 10.1007/s10295-014-1511-7

DO - 10.1007/s10295-014-1511-7

M3 - Article

VL - 41

SP - 1627

EP - 1636

JO - Journal of Industrial Microbiology and Biotechnology

T2 - Journal of Industrial Microbiology and Biotechnology

JF - Journal of Industrial Microbiology and Biotechnology

SN - 1367-5435

IS - 11

ER -