Polysaccharide determination in protein/polysaccharide mixtures for phase-diagram construction

Jacob K. Agbenorhevi, Vassilis Kontogiorgos

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of protein removal from protein-polysaccharide mixtures on the determination of total sugar concentration for phase-diagram construction was explored by response surface methodology. Phenol-sulphuric acid method was used to estimate the total sugar concentration whereas phase separation and microstructure of biopolymers in the mixtures were probed by means of fluorescent microscopy. All the factors studied (trifluoroacetic acid (TFA) volume, protein concentration, precipitation time) had significant influence on the polysaccharide recovery from the mixtures. The overall pattern of protein precipitation with TFA was also found to highly depend on protein-time interactions. Images taken at different mixture concentrations and with two different filter sets revealed a phase-separated system with the guar gum domains surrounded by a continuous protein phase. Experimental measurements as well as microscopical evidence suggests that, for greater than 60% polysaccharide recovery (±6% error), the protein content in the mixtures should be < ∼5.5% (w/v) with less than 0.5 mL TFA combined with more than 30 min rest under the influence of TFA. The results of the present investigation showed that when attempting to construct phase diagram of mixed biopolymer systems the equilibrium phases must be diluted to a protein content < ∼5% (w/v) before TFA treatment if the maximum polysaccharide concentration is to be determined. Crown

Original languageEnglish
Pages (from-to)849-854
Number of pages6
JournalCarbohydrate Polymers
Volume81
Issue number4
Early online date3 Apr 2010
DOIs
Publication statusPublished - 23 Jul 2010

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Polysaccharides
Phase diagrams
Trifluoroacetic acid
Trifluoroacetic Acid
Proteins
guar gum
Biopolymers
Sugars
Recovery
Phenol
Phase equilibria
Phase separation
Phenols
Microscopic examination
Microstructure
Acids

Cite this

Agbenorhevi, Jacob K. ; Kontogiorgos, Vassilis. / Polysaccharide determination in protein/polysaccharide mixtures for phase-diagram construction. In: Carbohydrate Polymers. 2010 ; Vol. 81, No. 4. pp. 849-854.
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Polysaccharide determination in protein/polysaccharide mixtures for phase-diagram construction. / Agbenorhevi, Jacob K.; Kontogiorgos, Vassilis.

In: Carbohydrate Polymers, Vol. 81, No. 4, 23.07.2010, p. 849-854.

Research output: Contribution to journalArticle

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