The aim of this qualitative study is to explore practice nurses’ perceptions of their role in providing diabetes care. Semi-structured interviews were conducted with 15 purposively selected general practice nurses working in the Northwest of England. Five main themes were identified through coding and demonstrate that definitions of diabetes service are complex and that variations exist in perceptions of care. The main findings give an insight into practice nurses’ perceptions of their scope and level of practice in primary diabetes care. This research highlights practice nurses’ training needs in order to meet new national guidelines in diabetes care.