Preparation and aqueous solution properties of thermoresponsive biocompatible AB diblock copolymers

Jeppe Madsen, Steven P. Armes, Karima Bertal, Sheila MacNeil, Andrew L. Lewis

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


The synthesis of a series of amphiphilic AB diblock copolymers in which the A block comprises poly(2-(methacryloyloxy)ethyl phosphorylcholine) [PMPC] and the B block comprises poly(2-hydroxypropyl methacrylate) [PHPMA] by atom transfer radical polymerization (ATRP) is reported. The aqueous solution properties of these new diblock copolymers were examined using dynamic light scattering and variable temperature 1H NMR spectroscopy. Copolymers with shorter thermoresponsive PHPMA blocks formed relatively large aggregates, while copolymers with longer PHPMA blocks formed smaller aggregates. This apparently "anomalous" self-assembly behavior occurs because the PHPMA block becomes more hydrophobic as its degree of polymerization is increased. Therefore, shorter PHPMA blocks lead to the formation of loose highly hydrated aggregates, whereas longer blocks formed more compact dehydrated aggregates. In addition, these new PMPC-PHPMA diblock copolymers are highly biocompatible and can mediate the relatively rapid efficient uptake of a fluorescent dye by human dermal fibroblast cells. Interestingly, dye uptake kinetics appear to depend on the hydrophobic/hydrophilic balance of the copolymer. This not only bodes well for in vitro imaging of live cells for biomedical applications but also highlights the importance of copolymer design to ensure efficient drug delivery.

Original languageEnglish
Pages (from-to)1875-1887
Number of pages13
Issue number7
Early online date5 Jun 2009
Publication statusPublished - 13 Jul 2009
Externally publishedYes

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