Presence of proline has a protective effect on weak acid stressed Saccharomyces cerevisiae

D. Greetham, H. Takagi, T. P. Phister

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fermentation of sugars released from lignocellulosic biomass (LCMs) is a sustainable option for the production of bioethanol. LCMs release fermentable hexose sugars and the currently non-fermentable pentose sugars; ethanol yield from lignocellulosic residues is dependent on the efficient conversion of available sugars to ethanol, a side-product of the process is acetic acid production. Presence of acetic acid reduced metabolic output and growth when compared with controls; however, it was observed that incubation with proline had a protective effect, which was proline specific and concentration dependent; the protective effect did not extend to furan or phenolic stressed yeast cells. Proline accumulating strains displayed tolerance to acetic acid when compared with background strains, whereas, strains with a compromised proline metabolism displayed sensitivity. Sensitivity to weak acids appears to be reduced with the addition of proline; proline is an imino acid freely available as a nitrogen source in the aerobic phase of fermentations. Yeast strains with higher intracellular proline concentrations would be desirable for industrial bioethanol fermentations.

Original languageEnglish
Pages (from-to)641-652
Number of pages12
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume105
Issue number4
Early online date6 Feb 2014
DOIs
Publication statusPublished - 1 Apr 2014
Externally publishedYes

Fingerprint Dive into the research topics of 'Presence of proline has a protective effect on weak acid stressed Saccharomyces cerevisiae'. Together they form a unique fingerprint.

  • Cite this