Male rape literature is limited in the UK, with little public attention and limited research about prison officers’ perceptions. The aim of the current study was to explore the attitudes and perceptions of prison officers in England and Wales. Two groups of participants were recruited: Group 1 included 24 retired prison officers; group 2 included 17 participants from the general population. A mixed method design was used to gather quantitative and qualitative data. Findings showed that prison officers did not accept societal myths on male rape, although some had negative attitudes towards male rape occurring in prison. Participants from the general population displayed similar results, demonstrating how being a prison officer does not affect beliefs and attitudes of male rape any more than the public. Support strategies for prisoners would further reduce the stigma of raped prisoners.