Probing the molecular determinants of coenzyme selectivity in the P450 BM3 FAD/NADPH domain

Keyphrases

• Nicotinamide Adenine Dinucleotide Phosphate (NADPH) 100%
• P450 BM3 100%
• Enzyme Selectivity 100%
• Molecular Determinants 100%
• FAD Domain 60%
• Point mutant 40%
• NAD(P) 20%
• Lysine 20%
• Efficiency Increase 20%
• Kinetic Study 20%
• Side Chain 20%
• Phosphate 20%
• Thermodynamic Properties 20%
• Kinetic Properties 20%
• Steady-state Flow 20%
• Optical Properties 20%
• Biotechnological Exploitation 20%
• Arginine 20%
• Kcat 20%
• NADH-dependent 20%
• Large Distances 20%
• Catalytic Efficiency 20%
• Double mutant 20%
• Bacillus Megaterium 20%
• Diflavin Reductase 20%
• Stopped-flow Kinetics 20%
• Specificity Constant 20%
• Conformational Influence 20%
• Isoalloxazine Ring 20%
• Selectivity Switch 20%
• Phosphate Binding 20%
• H-binding 20%

Biochemistry, Genetics and Molecular Biology

• Coenzyme 100%
• Cytochrome P450 100%
• Wild Type 75%
• Catalytic Efficiency 50%
• Oxidoreductase 25%
• Serine 25%
• Lysine 25%
• Reductase 25%
• Arginine 25%
• Binding Domain 25%
• Steady State 25%
• Bacillus Megaterium 25%
• Stopped-Flow Kinetics 25%
• Coenzyme NADH 25%
• Specificity Constant 25%

Pharmacology, Toxicology and Pharmaceutical Science

• Reduced Nicotinamide Adenine Dinucleotide Phosphate 100%
• Cytochrome P450 100%
• Oxidoreductase 20%
• Serine 20%
• Lysine 20%
• Bacillus Megaterium 20%
• Arginine 20%