Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotech

Subbroto Kumar Saha, Forhad Karim Saikot, Md Shahedur Rahman, Mohammad Abu Hena Mostofa Jamal, S. M.Khaledur Rahman, S. M.Riazul Islam, Ki Hyun Kim

Research output: Contribution to journalReview articlepeer-review

27 Citations (Scopus)

Abstract

Targeted genome editing is an advanced technique that enables precise modification of the nucleic acid sequences in a genome. Genome editing is typically performed using tools, such as molecular scissors, to cut a defined location in a specific gene. Genome editing has impacted various fields of biotechnology, such as agriculture; biopharmaceutical production; studies on the structure, regulation, and function of the genome; and the creation of transgenic organisms and cell lines. Although genome editing is used frequently, it has several limitations. Here, we provide an overview of well-studied genome-editing nucleases, including single-stranded oligodeoxynucleotides (ssODNs), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and CRISPR-Cas9 RNA-guided nucleases (CRISPR-Cas9). To this end, we describe the progress toward editable nuclease-based therapies and discuss the minimization of off-target mutagenesis. Future prospects of this challenging scientific field are also discussed.
Original languageEnglish
Pages (from-to)212-238
Number of pages27
JournalMolecular Therapy - Nucleic Acids
Volume14
Early online date17 Jan 2019
DOIs
Publication statusPublished - 1 Mar 2019
Externally publishedYes

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