Promoter Hypermethylation Mediates Downregulation of Thiamine Receptor SLC19A3 in Gastric Cancer

Xin Liu, Emily K.Y. Lam, Xian Wang, Jianbin Zhang, Yuen Yee Cheng, Yun W. Lam, Enders K.O. Ng, Jun Yu, Francis K.L. Chan, Hongchuan Jin, Joseph J.Y. Sung

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27 Citations (Scopus)


As an important way to inactivate tumor suppressor genes (TSGs) during cancer development, promoter hypermethylation can be used to define novel TSGs and identify biomarkers for cancer diagnosis. SLC19A3 (solute carrier family 19, member 3) was found to be such a biomarker. SLC19A3 expression was downregulated in gastric cancer cell lines (71%, 5/7) and restored after pharmacological demethylation. Notably, hypermethylation of SLC19A3 promoter was detected in gastric cancer cell lines (57%, 4/7), primary gastric carcinoma tissues (51%, 52/101) and precancerous lesion (intestinal metaplasia) tissues (32%, 8/25). Exogenous SLC19A3 expression caused growth inhibition of gastric cancer cells. In summary, SLC19A3 was epigenetically downregulated in gastric cancer. Methylation of SLC19A3 promoter could be a novel biomarker for early gastric cancer development.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalTumor Biology
Issue number5-6
Early online date7 Oct 2009
Publication statusPublished - 1 Dec 2009
Externally publishedYes

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