Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride

Kathrin Schedler, Ojan Assadian, Uta Brautferger, Gerald Müller, Torsten Koburger, Simon Classen, Axel Kramer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Currently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail. Methods: This proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results. Results: The difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy. Conclusion: Overall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.

Original languageEnglish
Article number143
JournalBMC Infectious Diseases
Volume17
Issue number1
DOIs
Publication statusPublished - 13 Feb 2017

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octenidine
Povidone-Iodine
Local Anti-Infective Agents
Wounds and Injuries
Exercise Test
Suspensions
Soil
Gels
Metals
Vantocil
In Vitro Techniques
chlorhexidine gluconate

Cite this

Schedler, Kathrin ; Assadian, Ojan ; Brautferger, Uta ; Müller, Gerald ; Koburger, Torsten ; Classen, Simon ; Kramer, Axel. / Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride. In: BMC Infectious Diseases. 2017 ; Vol. 17, No. 1.
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abstract = "Background: Currently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail. Methods: This proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results. Results: The difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy. Conclusion: Overall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.",
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Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride. / Schedler, Kathrin; Assadian, Ojan; Brautferger, Uta; Müller, Gerald; Koburger, Torsten; Classen, Simon; Kramer, Axel.

In: BMC Infectious Diseases, Vol. 17, No. 1, 143, 13.02.2017.

Research output: Contribution to journalArticle

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T1 - Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride

AU - Schedler, Kathrin

AU - Assadian, Ojan

AU - Brautferger, Uta

AU - Müller, Gerald

AU - Koburger, Torsten

AU - Classen, Simon

AU - Kramer, Axel

PY - 2017/2/13

Y1 - 2017/2/13

N2 - Background: Currently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail. Methods: This proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results. Results: The difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy. Conclusion: Overall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.

AB - Background: Currently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail. Methods: This proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results. Results: The difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy. Conclusion: Overall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.

KW - Bactericidal efficacy

KW - Chlorhexidine digluconate

KW - EN 14561

KW - Octenidine dihydrochloride

KW - Polyhexanide

KW - PVP-iodine

KW - Wound antisepsis

KW - Yeasticidal efficacy

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DO - 10.1186/s12879-017-2220-4

M3 - Article

VL - 17

JO - BMC Infectious Diseases

JF - BMC Infectious Diseases

SN - 1471-2334

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