Regulation of vitamin c and vitamin e on the biosynthesis of 1,3-propanediol by klebsiella pneumoniae

Yan Ping Zhang, Chen Yu Du, Zhi Rao, Zhu An Cao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glycerol could be transformed to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae. Since NADH was required in the process, reductants were considered to affect the formation of 1,3-propanediol. In this work, 40-150 mg/L VC or 20-100 mg/L VE was added into the culture medium to regulate the intracellular redox state (NADH/NAD) of Klebsiella pneumoniae, and the effects of these two reductants on the metabolism of Klebsiella pneumoniae were investigated. It was shown that the concentration of 1,3-propanediol increased by 20%-30% by addition of 150 mg/L VC or 30 mg/L VE into the culture medium. With the increasing 1,3-PD, some byproducts such as ethanol and 2,3-butanediol increased correspondingly, which resulted in an inconspicuous improvement of the yield of 1,3-PD. Rather than addition of these reductants, regulation of metabolic flux was considered to be a feasible attempt to improve the yield of 1,3-PD.

LanguageEnglish
Pages197-200
Number of pages4
JournalGuocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering
Volume5
Issue number2
Publication statusPublished - 1 Apr 2005
Externally publishedYes

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Vitamins
Biosynthesis
Glycerol
Metabolism
Byproducts
Ethanol
Fluxes
Reducing Agents
NAD
Culture Media
Oxidation-Reduction
1,3-propanediol

Cite this

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title = "Regulation of vitamin c and vitamin e on the biosynthesis of 1,3-propanediol by klebsiella pneumoniae",
abstract = "Glycerol could be transformed to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae. Since NADH was required in the process, reductants were considered to affect the formation of 1,3-propanediol. In this work, 40-150 mg/L VC or 20-100 mg/L VE was added into the culture medium to regulate the intracellular redox state (NADH/NAD) of Klebsiella pneumoniae, and the effects of these two reductants on the metabolism of Klebsiella pneumoniae were investigated. It was shown that the concentration of 1,3-propanediol increased by 20{\%}-30{\%} by addition of 150 mg/L VC or 30 mg/L VE into the culture medium. With the increasing 1,3-PD, some byproducts such as ethanol and 2,3-butanediol increased correspondingly, which resulted in an inconspicuous improvement of the yield of 1,3-PD. Rather than addition of these reductants, regulation of metabolic flux was considered to be a feasible attempt to improve the yield of 1,3-PD.",
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Regulation of vitamin c and vitamin e on the biosynthesis of 1,3-propanediol by klebsiella pneumoniae. / Zhang, Yan Ping; Du, Chen Yu; Rao, Zhi; Cao, Zhu An.

In: Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering, Vol. 5, No. 2, 01.04.2005, p. 197-200.

Research output: Contribution to journalArticle

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