RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection

Samantha Griffiths; Neil Portman; Philip R. Taylor; Siamon Gordon; Michael L. Ginger; Keith Gull

doi:10.1128/EC.00110-07

RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection

Samantha Griffiths, Neil Portman, Philip R. Taylor, Siamon Gordon, Michael L. Ginger, Keith Gull

University of Oxford

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.

Original language	English
Pages (from-to)	1248-1250
Number of pages	3
Journal	Eukaryotic Cell
Volume	6
Issue number	7
DOIs	https://doi.org/10.1128/EC.00110-07
Publication status	Published - 1 Jul 2007
Externally published	Yes

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title = "RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection",

abstract = "We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.",

author = "Samantha Griffiths and Neil Portman and Taylor, \{Philip R.\} and Siamon Gordon and Ginger, \{Michael L.\} and Keith Gull",

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T1 - RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection

AU - Griffiths, Samantha

AU - Portman, Neil

AU - Taylor, Philip R.

AU - Gordon, Siamon

AU - Ginger, Michael L.

AU - Gull, Keith

PY - 2007/7/1

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N2 - We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.

AB - We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.

UR - https://www.scopus.com/pages/publications/34447572946

U2 - 10.1128/EC.00110-07

DO - 10.1128/EC.00110-07

M3 - Article

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AN - SCOPUS:34447572946

SN - 1535-9778

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SP - 1248

EP - 1250

JO - Eukaryotic Cell

JF - Eukaryotic Cell

IS - 7

ER -

RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection

Abstract

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