Role of nanostructured lipid carriers in the expression alterations of ATP-binding cassette transporter genes in fluconazole-resistant Candida glabrata

Maryam  Moazeni; Majid Saeedi; Hamidreza Kelidari; Behrad Roohi; Mohammad T Hedayati; Tahereh  Shokohi; Mojtaba Nabili; Kofi Asare-Addo; Ali Nokhodchi

doi:10.34172/bi.2022.23825

Role of nanostructured lipid carriers in the expression alterations of ATP-binding cassette transporter genes in fluconazole-resistant Candida glabrata

Maryam Moazeni, Majid Saeedi, Hamidreza Kelidari, Behrad Roohi, Mohammad T Hedayati, Tahereh Shokohi, Mojtaba Nabili, Kofi Asare-Addo, Ali Nokhodchi

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: This study was proposed to assess the potential role of efflux transporters in reversing fluconazole resistance in Candida glabrata isolates treated with fluconazole loaded nanostructured lipid carriers (FLZ-NLCs).
Methods: The ultrasound technique was used to synthesize the FLZ-NLCs. Four fluconazole-resistant, as well as one susceptible standard C. glabrata isolates, were applied and exposed to FLZ/ FLZ-NLCs for 20 h at 37°C. Real-time PCRs were done to estimate the likely changes in ATP-binding cassette transporter genes.
Results: Similar to the FLZ-exposed-susceptible standard strain which showed no alteration, the genes were not up-regulated significantly under the FLZ-NLCs treated condition. While they were over-expressed when the yeasts were treated with fluconazole.
Conclusion: It is highly suggested that due to the nature of the NLCs which shields the whole conformation of the drug, FLZ is not recognized by the efflux transporter subunits and consequently the translocation would not happen.

Original language	English
Pages (from-to)	561-566
Number of pages	6
Journal	BioImpacts
Volume	12
Issue number	6
Early online date	30 Oct 2022
DOIs	https://doi.org/10.34172/bi.2022.23825
Publication status	Published - 1 Nov 2022

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@article{37227044ecf74b889f8dc53a29280ea1,

title = "Role of nanostructured lipid carriers in the expression alterations of ATP-binding cassette transporter genes in fluconazole-resistant Candida glabrata",

abstract = "Introduction: This study was proposed to assess the potential role of efflux transporters in reversing fluconazole resistance in Candida glabrata isolates treated with fluconazole loaded nanostructured lipid carriers (FLZ-NLCs).Methods: The ultrasound technique was used to synthesize the FLZ-NLCs. Four fluconazole-resistant, as well as one susceptible standard C. glabrata isolates, were applied and exposed to FLZ/ FLZ-NLCs for 20 h at 37°C. Real-time PCRs were done to estimate the likely changes in ATP-binding cassette transporter genes.Results: Similar to the FLZ-exposed-susceptible standard strain which showed no alteration, the genes were not up-regulated significantly under the FLZ-NLCs treated condition. While they were over-expressed when the yeasts were treated with fluconazole.Conclusion: It is highly suggested that due to the nature of the NLCs which shields the whole conformation of the drug, FLZ is not recognized by the efflux transporter subunits and consequently the translocation would not happen.",

keywords = "Fluconazole, Nanostructured lipid carriers, Candida glabrata, Resistance",

author = "Maryam Moazeni and Majid Saeedi and Hamidreza Kelidari and Behrad Roohi and Hedayati, {Mohammad T} and Tahereh Shokohi and Mojtaba Nabili and Kofi Asare-Addo and Ali Nokhodchi",

note = "Funding Information: The current research fund was granted by the MazUMS grant (No. 6325) received by MM (the first author). MazUMS had neither role in study design, nor data collection and interpretation, or the decision to submit the work for publication. Funding Information: We thank our funding corporation, Mazandaran University of Medical Sciences (MazUMS). The current research fund was granted by the MazUMS grant (No. 6325) received by MM (the first author). MazUMS had neither role in study design, nor data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} 2022 The Author(s).",

year = "2022",

month = nov,

day = "1",

doi = "10.34172/bi.2022.23825",

language = "English",

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pages = "561--566",

journal = "BioImpacts",

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number = "6",

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T1 - Role of nanostructured lipid carriers in the expression alterations of ATP-binding cassette transporter genes in fluconazole-resistant Candida glabrata

AU - Moazeni, Maryam

AU - Saeedi, Majid

AU - Kelidari, Hamidreza

AU - Roohi, Behrad

AU - Hedayati, Mohammad T

AU - Shokohi, Tahereh

AU - Nabili, Mojtaba

AU - Asare-Addo, Kofi

AU - Nokhodchi, Ali

N1 - Funding Information: The current research fund was granted by the MazUMS grant (No. 6325) received by MM (the first author). MazUMS had neither role in study design, nor data collection and interpretation, or the decision to submit the work for publication. Funding Information: We thank our funding corporation, Mazandaran University of Medical Sciences (MazUMS). The current research fund was granted by the MazUMS grant (No. 6325) received by MM (the first author). MazUMS had neither role in study design, nor data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: © 2022 The Author(s).

PY - 2022/11/1

Y1 - 2022/11/1

N2 - Introduction: This study was proposed to assess the potential role of efflux transporters in reversing fluconazole resistance in Candida glabrata isolates treated with fluconazole loaded nanostructured lipid carriers (FLZ-NLCs).Methods: The ultrasound technique was used to synthesize the FLZ-NLCs. Four fluconazole-resistant, as well as one susceptible standard C. glabrata isolates, were applied and exposed to FLZ/ FLZ-NLCs for 20 h at 37°C. Real-time PCRs were done to estimate the likely changes in ATP-binding cassette transporter genes.Results: Similar to the FLZ-exposed-susceptible standard strain which showed no alteration, the genes were not up-regulated significantly under the FLZ-NLCs treated condition. While they were over-expressed when the yeasts were treated with fluconazole.Conclusion: It is highly suggested that due to the nature of the NLCs which shields the whole conformation of the drug, FLZ is not recognized by the efflux transporter subunits and consequently the translocation would not happen.

AB - Introduction: This study was proposed to assess the potential role of efflux transporters in reversing fluconazole resistance in Candida glabrata isolates treated with fluconazole loaded nanostructured lipid carriers (FLZ-NLCs).Methods: The ultrasound technique was used to synthesize the FLZ-NLCs. Four fluconazole-resistant, as well as one susceptible standard C. glabrata isolates, were applied and exposed to FLZ/ FLZ-NLCs for 20 h at 37°C. Real-time PCRs were done to estimate the likely changes in ATP-binding cassette transporter genes.Results: Similar to the FLZ-exposed-susceptible standard strain which showed no alteration, the genes were not up-regulated significantly under the FLZ-NLCs treated condition. While they were over-expressed when the yeasts were treated with fluconazole.Conclusion: It is highly suggested that due to the nature of the NLCs which shields the whole conformation of the drug, FLZ is not recognized by the efflux transporter subunits and consequently the translocation would not happen.

KW - Fluconazole

KW - Nanostructured lipid carriers

KW - Candida glabrata

KW - Resistance

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DO - 10.34172/bi.2022.23825

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JO - BioImpacts

JF - BioImpacts

SN - 2228-5652

IS - 6

ER -

Role of nanostructured lipid carriers in the expression alterations of ATP-binding cassette transporter genes in fluconazole-resistant Candida glabrata

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