Role of phosphatidylinositol 3-kinase and specific protein kinase B isoforms in the suppression of apoptosis mediated by the Abelson protein- tyrosine kinase

Xiuwen Tang, C. Peter Downes, Anthony D. Whetton, P. Jane Owen-Lynch

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Leukemogenic oncogenes, such as the Abelson protein-tyrosine kinases (PTK), disrupt the normal regulation of survival, proliferation, and differentiation in hemopoietic progenitor cells. In the absence of cytokines, hemopoietic progenitor cells die by apoptosis. Abl PTKs mediate suppression of this apoptotic response leading to aberrant survival. To investigate the mechanism of Abl PTK action, we have used an interleukin-3-dependent murine mast cell line that expresses a temperature-sensitive form of the v-ABL PTK, which is active at the permissive temperature of 32 °C and inactive at 39 °C. At the permissive temperature, these cells are resistant to apoptosis induced both by the withdrawal of the hemopoietic growth factor (interleukin- 3) and the addition of cytotoxic drugs. We demonstrate that v-Abl associates with and stimulates activation of phosphatidylinositol 3-kinase (PI3K) and, crucially, that this activation results in enhanced cellular levels of the mass of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Activation of PI3K leads to enhanced activity of PKB and increased levels of the anti-apoptotic protein BclX(L). Transfection of cells with a dominant negative PKB reduces both the Abl-stimulated PKB activity and the survival effect conferred by activation of this oncogene. Thus, PI3K and PKB are required for the anti-apoptotic effects of Abl PTK.

Original languageEnglish
Pages (from-to)13142-13148
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number17
DOIs
Publication statusPublished - 28 Apr 2000
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-akt
Protein-Tyrosine Kinases
Protein Isoforms
Chemical activation
Apoptosis
Interleukin-3
Oncogenes
Temperature
Stem Cells
Apoptosis Regulatory Proteins
Second Messenger Systems
Mast Cells
Transfection
Intercellular Signaling Peptides and Proteins
Cells
Cytokines
Cell Line
Pharmaceutical Preparations

Cite this

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title = "Role of phosphatidylinositol 3-kinase and specific protein kinase B isoforms in the suppression of apoptosis mediated by the Abelson protein- tyrosine kinase",
abstract = "Leukemogenic oncogenes, such as the Abelson protein-tyrosine kinases (PTK), disrupt the normal regulation of survival, proliferation, and differentiation in hemopoietic progenitor cells. In the absence of cytokines, hemopoietic progenitor cells die by apoptosis. Abl PTKs mediate suppression of this apoptotic response leading to aberrant survival. To investigate the mechanism of Abl PTK action, we have used an interleukin-3-dependent murine mast cell line that expresses a temperature-sensitive form of the v-ABL PTK, which is active at the permissive temperature of 32 °C and inactive at 39 °C. At the permissive temperature, these cells are resistant to apoptosis induced both by the withdrawal of the hemopoietic growth factor (interleukin- 3) and the addition of cytotoxic drugs. We demonstrate that v-Abl associates with and stimulates activation of phosphatidylinositol 3-kinase (PI3K) and, crucially, that this activation results in enhanced cellular levels of the mass of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Activation of PI3K leads to enhanced activity of PKB and increased levels of the anti-apoptotic protein BclX(L). Transfection of cells with a dominant negative PKB reduces both the Abl-stimulated PKB activity and the survival effect conferred by activation of this oncogene. Thus, PI3K and PKB are required for the anti-apoptotic effects of Abl PTK.",
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Role of phosphatidylinositol 3-kinase and specific protein kinase B isoforms in the suppression of apoptosis mediated by the Abelson protein- tyrosine kinase. / Tang, Xiuwen; Downes, C. Peter; Whetton, Anthony D.; Owen-Lynch, P. Jane.

In: Journal of Biological Chemistry, Vol. 275, No. 17, 28.04.2000, p. 13142-13148.

Research output: Contribution to journalArticle

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