Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles

Donny van der Meer, Mari L Uchimoto, Graham Williams

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and the identity of the body fluid using a single process. DNA and micro-RNA were extracted from blood and saliva before undergoing a cDNA synthesis step by using stem-loop reverse transcription PCR. The resulting extracts containing DNA and cDNA synthesized from body fluid-specific miRNA markers then underwent standard STR analysis using a modified ABI AmpFℓSTR(®) NGM SElect™ kit. In all samples, a full DNA profile was obtained along with additional peaks corresponding to the miRNA marker targeted. In all cases, blood samples profiled exhibited a peak indicating the presence of the blood-specific miRNA marker and the saliva sample profiled exhibited a peak indicating the presence of the saliva-specific miRNA marker.

Original languageEnglish
Pages (from-to)967-71
Number of pages5
JournalJournal of Forensic Sciences
Volume58
Issue number4
Early online date17 May 2013
DOIs
Publication statusPublished - 9 Jul 2013

Fingerprint

Body Fluids
MicroRNAs
Saliva
DNA
Complementary DNA
Reverse Transcription
Polymerase Chain Reaction

Cite this

van der Meer, Donny ; Uchimoto, Mari L ; Williams, Graham. / Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles. In: Journal of Forensic Sciences. 2013 ; Vol. 58, No. 4. pp. 967-71.
@article{4d38ca0311aa4977af9e4abc5bee20f4,
title = "Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles",
abstract = "Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and the identity of the body fluid using a single process. DNA and micro-RNA were extracted from blood and saliva before undergoing a cDNA synthesis step by using stem-loop reverse transcription PCR. The resulting extracts containing DNA and cDNA synthesized from body fluid-specific miRNA markers then underwent standard STR analysis using a modified ABI AmpFℓSTR({\circledR}) NGM SElect™ kit. In all samples, a full DNA profile was obtained along with additional peaks corresponding to the miRNA marker targeted. In all cases, blood samples profiled exhibited a peak indicating the presence of the blood-specific miRNA marker and the saliva sample profiled exhibited a peak indicating the presence of the saliva-specific miRNA marker.",
keywords = "Blood Chemical Analysis, DNA, DNA Fingerprinting, Electrophoresis, Capillary, Humans, MicroRNAs, Microsatellite Repeats, Real-Time Polymerase Chain Reaction, Reverse Transcription, Saliva, Journal Article, Research Support, Non-U.S. Gov't",
author = "{van der Meer}, Donny and Uchimoto, {Mari L} and Graham Williams",
note = "{\circledC} 2013 American Academy of Forensic Sciences.",
year = "2013",
month = "7",
day = "9",
doi = "10.1111/1556-4029.12160",
language = "English",
volume = "58",
pages = "967--71",
journal = "Journal of Forensic Sciences",
issn = "0022-1198",
publisher = "Wiley-Blackwell",
number = "4",

}

Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles. / van der Meer, Donny; Uchimoto, Mari L; Williams, Graham.

In: Journal of Forensic Sciences, Vol. 58, No. 4, 09.07.2013, p. 967-71.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles

AU - van der Meer, Donny

AU - Uchimoto, Mari L

AU - Williams, Graham

N1 - © 2013 American Academy of Forensic Sciences.

PY - 2013/7/9

Y1 - 2013/7/9

N2 - Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and the identity of the body fluid using a single process. DNA and micro-RNA were extracted from blood and saliva before undergoing a cDNA synthesis step by using stem-loop reverse transcription PCR. The resulting extracts containing DNA and cDNA synthesized from body fluid-specific miRNA markers then underwent standard STR analysis using a modified ABI AmpFℓSTR(®) NGM SElect™ kit. In all samples, a full DNA profile was obtained along with additional peaks corresponding to the miRNA marker targeted. In all cases, blood samples profiled exhibited a peak indicating the presence of the blood-specific miRNA marker and the saliva sample profiled exhibited a peak indicating the presence of the saliva-specific miRNA marker.

AB - Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and the identity of the body fluid using a single process. DNA and micro-RNA were extracted from blood and saliva before undergoing a cDNA synthesis step by using stem-loop reverse transcription PCR. The resulting extracts containing DNA and cDNA synthesized from body fluid-specific miRNA markers then underwent standard STR analysis using a modified ABI AmpFℓSTR(®) NGM SElect™ kit. In all samples, a full DNA profile was obtained along with additional peaks corresponding to the miRNA marker targeted. In all cases, blood samples profiled exhibited a peak indicating the presence of the blood-specific miRNA marker and the saliva sample profiled exhibited a peak indicating the presence of the saliva-specific miRNA marker.

KW - Blood Chemical Analysis

KW - DNA

KW - DNA Fingerprinting

KW - Electrophoresis, Capillary

KW - Humans

KW - MicroRNAs

KW - Microsatellite Repeats

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcription

KW - Saliva

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1556-4029

U2 - 10.1111/1556-4029.12160

DO - 10.1111/1556-4029.12160

M3 - Article

VL - 58

SP - 967

EP - 971

JO - Journal of Forensic Sciences

JF - Journal of Forensic Sciences

SN - 0022-1198

IS - 4

ER -