Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles

Donny van der Meer, Mari L Uchimoto, Graham Williams

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and the identity of the body fluid using a single process. DNA and micro-RNA were extracted from blood and saliva before undergoing a cDNA synthesis step by using stem-loop reverse transcription PCR. The resulting extracts containing DNA and cDNA synthesized from body fluid-specific miRNA markers then underwent standard STR analysis using a modified ABI AmpFℓSTR(®) NGM SElect™ kit. In all samples, a full DNA profile was obtained along with additional peaks corresponding to the miRNA marker targeted. In all cases, blood samples profiled exhibited a peak indicating the presence of the blood-specific miRNA marker and the saliva sample profiled exhibited a peak indicating the presence of the saliva-specific miRNA marker.

Original languageEnglish
Pages (from-to)967-71
Number of pages5
JournalJournal of Forensic Sciences
Volume58
Issue number4
Early online date17 May 2013
DOIs
Publication statusPublished - 9 Jul 2013

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