Simultaneous determination of cereal monosaccharides, xylo- and arabinoxylooligosaccharides and uronic acids using HPAEC-PAD

Mohammad Alyassin, Grant Campbell, Helen Masey O’Neil, Michael Bedford

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Xylo- and arabinoxylo-oligosaccharides (XOS and AXOS) are of interest for their prebiotic activity. The production of these oligomers might be accompanied with monosaccharides. The measurement of both oligosaccharides and monosaccharides usually requires two methods. The current work presents an HPAEC-PAD method based on gradient elution of aqueous solvents sodium hydroxide and sodium acetate, in contrast to conventional isocratic elution, for the simultaneous separation of 16 standards of monosaccharides, xylo-oligosaccharides, arabinoxylo-oligosaccharides and uronic acids using CarboPac PA 200 column. The presented method showed a stable baseline and high-resolution separation of the standards. The method showed acceptable accuracy and precision. Limits of Detection and Quantitation (LOD and LOQ) were estimated for all the standards. The method was applied to measure the activity of a commercial endoxylanase on wheat bran; a steady release of xylose monosaccharide was observed. Enzyme action on oligosaccharide standards showed a preference for the larger oligosaccharides.
Original languageEnglish
Article number126221
Number of pages8
JournalFood Chemistry
Volume315
Early online date16 Jan 2020
DOIs
Publication statusPublished - 15 Jun 2020

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