Single Step Assembly of Biomolecule-loaded Sub-micron Polysulfone Fibers

B. Ghorani, Stephen J. Russell, Andrew Hebden, Parikshit Goswami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Enrichment of chemically resistant hydrophobic polymers with polar biomolecules is relevant to the production of fiber-based drug delivery devices and adsorptive filtration media, as well as fibers for selective molecular recognition of antibodies, enzymes and nucleic acids. Polysulfone (PSU) is an amorphous polymer possessing high-strength, rigidity and excellent thermal stability. The preparation of PSU spinning solutions requires lengthy dissolution times at elevated temperature that tends to degrade commixed polar biomolecules. Using the highly polar metabolite creatinine, as a model system, a variety of co-solvents was evaluated for electrospinning commixed solutions of PSU and creatinine at room temperature. The selection of solvent systems was informed by Hansen solubility parameters. A binary system of N, N-dimethylacetamide (DMAc):methanol (4:1) was not found to be a suitable solvent because of the need for elevated temperature (80℃) to facilitate dissolution, and a binary solvent system of N, N-dimethylformamide (DMF):dimethyl sulfoxide (DMSO) (3:2) resulted in nozzle blockage during spinning. A binary system of DMAc:DMSO (13:7) enabled preparation of PSU with creatinine at ambient temperature, and sub-micron fibers substantially free of beads were produced continuously via electrospinning, yielding fiber diameters in the range 470–870 nm. The presence of creatinine was confirmed by high performance liquid chromatography (HPLC), and fiber morphology was examined by scanning electron microscopy (SEM).
LanguageEnglish
Pages340-350
Number of pages11
JournalTextile Reseach Journal
Volume87
Issue number3
Early online date21 Jun 2016
DOIs
Publication statusPublished - 1 Feb 2017
Externally publishedYes

Fingerprint

Polysulfones
Biomolecules
Creatinine
Fibers
Dimethyl sulfoxide
Electrospinning
Dimethyl Sulfoxide
Polymers
Dissolution
Dimethylformamide
Molecular recognition
Temperature
Nucleic acids
High performance liquid chromatography
Metabolites
Drug delivery
Antibodies
Rigidity
Nucleic Acids
Methanol

Cite this

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title = "Single Step Assembly of Biomolecule-loaded Sub-micron Polysulfone Fibers",
abstract = "Enrichment of chemically resistant hydrophobic polymers with polar biomolecules is relevant to the production of fiber-based drug delivery devices and adsorptive filtration media, as well as fibers for selective molecular recognition of antibodies, enzymes and nucleic acids. Polysulfone (PSU) is an amorphous polymer possessing high-strength, rigidity and excellent thermal stability. The preparation of PSU spinning solutions requires lengthy dissolution times at elevated temperature that tends to degrade commixed polar biomolecules. Using the highly polar metabolite creatinine, as a model system, a variety of co-solvents was evaluated for electrospinning commixed solutions of PSU and creatinine at room temperature. The selection of solvent systems was informed by Hansen solubility parameters. A binary system of N, N-dimethylacetamide (DMAc):methanol (4:1) was not found to be a suitable solvent because of the need for elevated temperature (80℃) to facilitate dissolution, and a binary solvent system of N, N-dimethylformamide (DMF):dimethyl sulfoxide (DMSO) (3:2) resulted in nozzle blockage during spinning. A binary system of DMAc:DMSO (13:7) enabled preparation of PSU with creatinine at ambient temperature, and sub-micron fibers substantially free of beads were produced continuously via electrospinning, yielding fiber diameters in the range 470–870 nm. The presence of creatinine was confirmed by high performance liquid chromatography (HPLC), and fiber morphology was examined by scanning electron microscopy (SEM).",
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author = "B. Ghorani and Russell, {Stephen J.} and Andrew Hebden and Parikshit Goswami",
note = "No full text in Eprints. Can't find the accepted date that has been entered so not sure if this is accurate. If it is correct then it is pre 1st April 2016 and does not need to be OA compliant. CC belongs to the authors, but there is no information on what kind of copyright it is in terms of open access. HN 02/11/2017",
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Single Step Assembly of Biomolecule-loaded Sub-micron Polysulfone Fibers. / Ghorani, B.; Russell, Stephen J.; Hebden, Andrew; Goswami, Parikshit.

In: Textile Reseach Journal, Vol. 87, No. 3, 01.02.2017, p. 340-350.

Research output: Contribution to journalArticle

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AU - Russell, Stephen J.

AU - Hebden, Andrew

AU - Goswami, Parikshit

N1 - No full text in Eprints. Can't find the accepted date that has been entered so not sure if this is accurate. If it is correct then it is pre 1st April 2016 and does not need to be OA compliant. CC belongs to the authors, but there is no information on what kind of copyright it is in terms of open access. HN 02/11/2017

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Enrichment of chemically resistant hydrophobic polymers with polar biomolecules is relevant to the production of fiber-based drug delivery devices and adsorptive filtration media, as well as fibers for selective molecular recognition of antibodies, enzymes and nucleic acids. Polysulfone (PSU) is an amorphous polymer possessing high-strength, rigidity and excellent thermal stability. The preparation of PSU spinning solutions requires lengthy dissolution times at elevated temperature that tends to degrade commixed polar biomolecules. Using the highly polar metabolite creatinine, as a model system, a variety of co-solvents was evaluated for electrospinning commixed solutions of PSU and creatinine at room temperature. The selection of solvent systems was informed by Hansen solubility parameters. A binary system of N, N-dimethylacetamide (DMAc):methanol (4:1) was not found to be a suitable solvent because of the need for elevated temperature (80℃) to facilitate dissolution, and a binary solvent system of N, N-dimethylformamide (DMF):dimethyl sulfoxide (DMSO) (3:2) resulted in nozzle blockage during spinning. A binary system of DMAc:DMSO (13:7) enabled preparation of PSU with creatinine at ambient temperature, and sub-micron fibers substantially free of beads were produced continuously via electrospinning, yielding fiber diameters in the range 470–870 nm. The presence of creatinine was confirmed by high performance liquid chromatography (HPLC), and fiber morphology was examined by scanning electron microscopy (SEM).

AB - Enrichment of chemically resistant hydrophobic polymers with polar biomolecules is relevant to the production of fiber-based drug delivery devices and adsorptive filtration media, as well as fibers for selective molecular recognition of antibodies, enzymes and nucleic acids. Polysulfone (PSU) is an amorphous polymer possessing high-strength, rigidity and excellent thermal stability. The preparation of PSU spinning solutions requires lengthy dissolution times at elevated temperature that tends to degrade commixed polar biomolecules. Using the highly polar metabolite creatinine, as a model system, a variety of co-solvents was evaluated for electrospinning commixed solutions of PSU and creatinine at room temperature. The selection of solvent systems was informed by Hansen solubility parameters. A binary system of N, N-dimethylacetamide (DMAc):methanol (4:1) was not found to be a suitable solvent because of the need for elevated temperature (80℃) to facilitate dissolution, and a binary solvent system of N, N-dimethylformamide (DMF):dimethyl sulfoxide (DMSO) (3:2) resulted in nozzle blockage during spinning. A binary system of DMAc:DMSO (13:7) enabled preparation of PSU with creatinine at ambient temperature, and sub-micron fibers substantially free of beads were produced continuously via electrospinning, yielding fiber diameters in the range 470–870 nm. The presence of creatinine was confirmed by high performance liquid chromatography (HPLC), and fiber morphology was examined by scanning electron microscopy (SEM).

KW - Electrospinning

KW - Nanofibers

KW - Polysulfone

KW - Biomolecule

KW - Creatinine

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SN - 0040-5175

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