Solution properties of capsular polysaccharides from Streptococcus pneumoniae

Stephen E. Harding; Ali Saber Abdelhameed; Gordon A. Morris; Gary Adams; Olivier Laloux; Louis Cerny; Benjamin Bonnier; Pierre Duvivier; Karel Conrath; Christophe Lenfant

doi:10.1016/j.carbpol.2012.05.030

Solution properties of capsular polysaccharides from Streptococcus pneumoniae

Stephen E. Harding, Ali Saber Abdelhameed, Gordon A. Morris, Gary Adams, Olivier Laloux, Louis Cerny, Benjamin Bonnier, Pierre Duvivier, Karel Conrath, Christophe Lenfant

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been studied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths L _p), important properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering measurements) were generally unimodal, with weight (mass) average molar masses ranging from 100 × 10 ³ to 1300 × 10 ³ g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ∼4 to 9 nm.

Original language	English
Pages (from-to)	237-242
Number of pages	6
Journal	Carbohydrate Polymers
Volume	90
Issue number	1
Early online date	15 May 2012
DOIs	https://doi.org/10.1016/j.carbpol.2012.05.030
Publication status	Published - 1 Sep 2012
Externally published	Yes

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abstract = "Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been studied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths L p), important properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering measurements) were generally unimodal, with weight (mass) average molar masses ranging from 100 × 10 3 to 1300 × 10 3 g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ∼4 to 9 nm.",

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AU - Harding, Stephen E.

AU - Abdelhameed, Ali Saber

AU - Morris, Gordon A.

AU - Adams, Gary

AU - Laloux, Olivier

AU - Cerny, Louis

AU - Bonnier, Benjamin

AU - Duvivier, Pierre

AU - Conrath, Karel

AU - Lenfant, Christophe

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AB - Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been studied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths L p), important properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering measurements) were generally unimodal, with weight (mass) average molar masses ranging from 100 × 10 3 to 1300 × 10 3 g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ∼4 to 9 nm.

KW - Bacterial

KW - Conformation

KW - Hydrodynamics

KW - Size distribution

KW - Vaccines

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JO - Carbohydrate Polymers

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ER -

Solution properties of capsular polysaccharides from Streptococcus pneumoniae

Abstract

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