Solution properties of capsular polysaccharides from Streptococcus pneumoniae

Stephen E. Harding, Ali Saber Abdelhameed, Gordon A. Morris, Gary Adams, Olivier Laloux, Louis Cerny, Benjamin Bonnier, Pierre Duvivier, Karel Conrath, Christophe Lenfant

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been studied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths L p), important properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering measurements) were generally unimodal, with weight (mass) average molar masses ranging from 100 × 10 3 to 1300 × 10 3 g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ∼4 to 9 nm.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalCarbohydrate Polymers
Volume90
Issue number1
Early online date15 May 2012
DOIs
Publication statusPublished - 1 Sep 2012
Externally publishedYes

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Molar mass
Polysaccharides
Streptococcus pneumoniae
Sedimentation
Glycoconjugates
Hydrodynamics
Viscosity
Gel Chromatography
Vaccines
Flexible structures
Size exclusion chromatography
Light scattering
Conformations
Light
Weights and Measures
Serogroup

Cite this

Harding, S. E., Abdelhameed, A. S., Morris, G. A., Adams, G., Laloux, O., Cerny, L., ... Lenfant, C. (2012). Solution properties of capsular polysaccharides from Streptococcus pneumoniae. Carbohydrate Polymers, 90(1), 237-242. https://doi.org/10.1016/j.carbpol.2012.05.030
Harding, Stephen E. ; Abdelhameed, Ali Saber ; Morris, Gordon A. ; Adams, Gary ; Laloux, Olivier ; Cerny, Louis ; Bonnier, Benjamin ; Duvivier, Pierre ; Conrath, Karel ; Lenfant, Christophe. / Solution properties of capsular polysaccharides from Streptococcus pneumoniae. In: Carbohydrate Polymers. 2012 ; Vol. 90, No. 1. pp. 237-242.
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Harding, SE, Abdelhameed, AS, Morris, GA, Adams, G, Laloux, O, Cerny, L, Bonnier, B, Duvivier, P, Conrath, K & Lenfant, C 2012, 'Solution properties of capsular polysaccharides from Streptococcus pneumoniae', Carbohydrate Polymers, vol. 90, no. 1, pp. 237-242. https://doi.org/10.1016/j.carbpol.2012.05.030

Solution properties of capsular polysaccharides from Streptococcus pneumoniae. / Harding, Stephen E.; Abdelhameed, Ali Saber; Morris, Gordon A.; Adams, Gary; Laloux, Olivier; Cerny, Louis; Bonnier, Benjamin; Duvivier, Pierre; Conrath, Karel; Lenfant, Christophe.

In: Carbohydrate Polymers, Vol. 90, No. 1, 01.09.2012, p. 237-242.

Research output: Contribution to journalArticle

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