Sperm structure of Limoniidae and their phylogenetic relationship with Tipulidae (Diptera, Nematocera)

Romano Dallai, Bianca Maria Lombardo, David Mercati, Stefano Vanin, Pietro Lupetti

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The sperm ultrastructure of a few species of Limoniidae (Limonia nigropunctata; L. nubeculosa; Chionea n. sp.; C. alpina; C. lutescens) was studied. The two species of Limonia have a monolayered acrosome with crystallized material, a three-lobed nucleus in cross section, a ring of centriole adjunct material and a flagellum which consists of a 9 + 9 + 1 axoneme and a single mitochondrial derivative. The central axonemal tubule is provided with 15 protofilaments in its tubular wall, while the accessory tubules have 13 protofilaments and are flanked by the electron-dense intertubular material. The three species of Chionea share a monolayered acrosome, a nucleus with two longitudinal grooves, a centriole adjunct material which surrounds the centriole and the initial part of the axoneme. The axoneme is of conventional type, with 9 + 9 + 2 microtubular pattern, with accessory tubules provided with 13 protofilaments and intertubular material. However, in C. lutescens the accessory tubules start with 15 protofilaments and transform into a tubule with 13 protofilaments. These data are discussed in the light of the phylogenetic relationship between Limoniidae and Tipulidae. For this purpose, the sperm ultrastructure of Nephrotoma appendiculata was also considered comparatively.

LanguageEnglish
Pages81-92
Number of pages12
JournalArthropod Structure and Development
Volume37
Issue number1
Early online date18 May 2007
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Fingerprint

Limoniidae
Tipulidae
Axoneme
Centrioles
Plumbaginaceae
Diptera
sperm
Spermatozoa
Acrosome
centrioles
spermatozoa
phylogenetics
Limonia
phylogeny
acrosome
ultrastructure
Flagella
Electrons
flagellum
transform

Cite this

Dallai, Romano ; Lombardo, Bianca Maria ; Mercati, David ; Vanin, Stefano ; Lupetti, Pietro. / Sperm structure of Limoniidae and their phylogenetic relationship with Tipulidae (Diptera, Nematocera). In: Arthropod Structure and Development. 2008 ; Vol. 37, No. 1. pp. 81-92.
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Sperm structure of Limoniidae and their phylogenetic relationship with Tipulidae (Diptera, Nematocera). / Dallai, Romano; Lombardo, Bianca Maria; Mercati, David; Vanin, Stefano; Lupetti, Pietro.

In: Arthropod Structure and Development, Vol. 37, No. 1, 01.2008, p. 81-92.

Research output: Contribution to journalArticle

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T1 - Sperm structure of Limoniidae and their phylogenetic relationship with Tipulidae (Diptera, Nematocera)

AU - Dallai, Romano

AU - Lombardo, Bianca Maria

AU - Mercati, David

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AU - Lupetti, Pietro

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AB - The sperm ultrastructure of a few species of Limoniidae (Limonia nigropunctata; L. nubeculosa; Chionea n. sp.; C. alpina; C. lutescens) was studied. The two species of Limonia have a monolayered acrosome with crystallized material, a three-lobed nucleus in cross section, a ring of centriole adjunct material and a flagellum which consists of a 9 + 9 + 1 axoneme and a single mitochondrial derivative. The central axonemal tubule is provided with 15 protofilaments in its tubular wall, while the accessory tubules have 13 protofilaments and are flanked by the electron-dense intertubular material. The three species of Chionea share a monolayered acrosome, a nucleus with two longitudinal grooves, a centriole adjunct material which surrounds the centriole and the initial part of the axoneme. The axoneme is of conventional type, with 9 + 9 + 2 microtubular pattern, with accessory tubules provided with 13 protofilaments and intertubular material. However, in C. lutescens the accessory tubules start with 15 protofilaments and transform into a tubule with 13 protofilaments. These data are discussed in the light of the phylogenetic relationship between Limoniidae and Tipulidae. For this purpose, the sperm ultrastructure of Nephrotoma appendiculata was also considered comparatively.

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