Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition

Elisa C. Ale, Virginia A. Batistela, Gabriela Correa Olivar, Joana B. Ferrado, Sohaib Sadiq, Hafiz I. Ahmed, Jorge A. Reinheimer, Luciana Vera-Candioti, Andrew P. Laws, Ana Griselda Binetti

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Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semidefined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterisation indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.

Original languageEnglish
Pages (from-to)76-87
Number of pages12
JournalInternational Journal of Dairy Technology
Issue number1
Early online date21 Aug 2019
Publication statusPublished - 1 Feb 2020


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