Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition

Elisa C. Ale, Virginia A. Batistela, Gabriela Correa Olivar, Joana B. Ferrado, Sohaib Sadiq, Hafiz I. Ahmed, Jorge A. Reinheimer, Luciana Vera-Candioti, Andrew P. Laws, Ana Griselda Binetti

Research output: Contribution to journalArticle

Abstract

Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semidefined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterisation indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.

LanguageEnglish
JournalInternational Journal of Dairy Technology
Early online date21 Aug 2019
DOIs
Publication statusE-pub ahead of print - 21 Aug 2019

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Lactobacillus fermentum
beta-Glucans
exopolysaccharides
Molecular mass
chemical composition
Mass Media
Dairies
Sugar (sucrose)
beta-glucans
Lactic acid
Chemical analysis
Yeast
Sucrose
Lactic Acid
Bacteria
Nitrogen
Yeasts
molecular weight
Food
Molecules

Cite this

Ale, Elisa C. ; Batistela, Virginia A. ; Correa Olivar, Gabriela ; Ferrado, Joana B. ; Sadiq, Sohaib ; Ahmed, Hafiz I. ; Reinheimer, Jorge A. ; Vera-Candioti, Luciana ; Laws, Andrew P. ; Binetti, Ana Griselda. / Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition. In: International Journal of Dairy Technology. 2019.
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abstract = "Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semidefined medium (SDM, 0.63{\%} yeast nitrogen base, 0.53{\%} bacto casitone, 0.53{\%} ammonium citrate, 6.25{\%} sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterisation indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.",
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Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition. / Ale, Elisa C.; Batistela, Virginia A.; Correa Olivar, Gabriela; Ferrado, Joana B.; Sadiq, Sohaib; Ahmed, Hafiz I.; Reinheimer, Jorge A.; Vera-Candioti, Luciana; Laws, Andrew P.; Binetti, Ana Griselda.

In: International Journal of Dairy Technology, 21.08.2019.

Research output: Contribution to journalArticle

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AU - Ale, Elisa C.

AU - Batistela, Virginia A.

AU - Correa Olivar, Gabriela

AU - Ferrado, Joana B.

AU - Sadiq, Sohaib

AU - Ahmed, Hafiz I.

AU - Reinheimer, Jorge A.

AU - Vera-Candioti, Luciana

AU - Laws, Andrew P.

AU - Binetti, Ana Griselda

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AB - Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β-glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semidefined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterisation indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β-glucan.

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