Strength of Axial Water Ligation in Substrate-Free Cytochrome P450s is Isoform Dependent

Kip P. Conner, Alina M. Schimpf, Alex A. Cruce, Kirsty J. McLean, Andrew W. Munro, Daniel J. Frank, Matthew D. Krzyaniak, Paul Ortiz De Montellano, Michael K. Bowman, William M. Atkins

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Abstract

The heme-containing cytochrome P450s exhibit isoform-dependent ferric spin equilibria in the resting state and differential substrate-dependent spin equilibria. The basis for these differences is not well understood. Here, magnetic circular dichroism (MCD) reveals significant differences in the resting low spin ligand field of CYPs 3A4, 2E1, 2C9, 125A1, and 51B1, which indicates differences in the strength of axial water ligation to the heme. The near-infrared bands that specifically correspond to charge-transfer porphyrin-to-metal transitions span a range of energies of nearly 2 kcal/mol. In addition, the experimentally determined MCD bands are not entirely in agreement with the expected MCD energies calculated from electron paramagnetic resonance parameters, thus emphasizing the need for the experimental data. MCD marker bands of the high spin heme between 500 and 680 nm were also measured and suggest only a narrow range of energies for this ensemble of high spin Cys(S-) → Fe3+ transitions among these isoforms. The differences in axial ligand energies between CYP isoforms of the low spin states likely contribute to the energetics of substrate-dependent spin state perturbation. However, these ligand field energies do not correlate with the fraction of high spin vs low spin in the resting state enzyme, suggestive of differences in water access to the heme or isoform-dependent differences in the substrate-free high spin states as well.

Original languageEnglish
Pages (from-to)1428-1434
Number of pages7
JournalBiochemistry
Volume53
Issue number9
Early online date20 Feb 2014
DOIs
Publication statusPublished - 11 Mar 2014
Externally publishedYes

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    Conner, K. P., Schimpf, A. M., Cruce, A. A., McLean, K. J., Munro, A. W., Frank, D. J., Krzyaniak, M. D., Ortiz De Montellano, P., Bowman, M. K., & Atkins, W. M. (2014). Strength of Axial Water Ligation in Substrate-Free Cytochrome P450s is Isoform Dependent. Biochemistry, 53(9), 1428-1434. https://doi.org/10.1021/bi401547j