TY - JOUR
T1 - Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases
AU - Canning, Peter
AU - Cooper, Christopher D O
AU - Krojer, Tobias
AU - Murray, James W.
AU - Pike, Ashley C W
AU - Chaikuad, Apirat
AU - Keates, Tracy
AU - Thangaratnarajah, Chancievan
AU - Hojzan, Viktorija
AU - Ayinampudi, Vikram
AU - Marsden, Brian D.
AU - Gileadi, Opher
AU - Knapp, Stefan
AU - Von Delft, Frank
AU - Bullock, Alex N.
N1 - See related notice of erratum: dx.doi.org/10.1074/jbc.A112.437996. Vikram Ayinampudi was omitted from the list of authors on original article but has been added to this record as per correction.
PY - 2013/3/15
Y1 - 2013/3/15
N2 - Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 assembly, and substrate recognition into a single multidomain protein, of which the best known are BTB-BACK-Kelch domain proteins, including KEAP1. Cul3 assembly requires a BTB protein "3-box" motif, analogous to the F-box and SOCS box motifs of other Cullin-based E3s. To define the molecular basis for this assembly and the overall architecture of the E3, we determined the crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3, along with the Kelch domain structures of KLHL2 (Mayven), KLHL7, KLHL12, and KBTBD5. We show that Cul3 interaction is dependent on a unique N-terminal extension sequence that packs against the 3-box in a hydrophobic groove centrally located between the BTB and BACK domains. Deletion of this N-terminal region results in a 30-fold loss in affinity. The presented data offer a model for the quaternary assembly of this E3 class that supports the bivalent capture of Nrf2 and reveals potential new sites for E3 inhibitor design.
AB - Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 assembly, and substrate recognition into a single multidomain protein, of which the best known are BTB-BACK-Kelch domain proteins, including KEAP1. Cul3 assembly requires a BTB protein "3-box" motif, analogous to the F-box and SOCS box motifs of other Cullin-based E3s. To define the molecular basis for this assembly and the overall architecture of the E3, we determined the crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3, along with the Kelch domain structures of KLHL2 (Mayven), KLHL7, KLHL12, and KBTBD5. We show that Cul3 interaction is dependent on a unique N-terminal extension sequence that packs against the 3-box in a hydrophobic groove centrally located between the BTB and BACK domains. Deletion of this N-terminal region results in a 30-fold loss in affinity. The presented data offer a model for the quaternary assembly of this E3 class that supports the bivalent capture of Nrf2 and reveals potential new sites for E3 inhibitor design.
UR - http://www.scopus.com/inward/record.url?scp=84875152063&partnerID=8YFLogxK
UR - http://www.jbc.org/
UR - http://www.jbc.org/content/288/39/28304
U2 - 10.1074/jbc.M112.437996
DO - 10.1074/jbc.M112.437996
M3 - Article
C2 - 23349464
AN - SCOPUS:84875152063
VL - 288
SP - 7803
EP - 7814
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 11
ER -