Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases

Peter Canning, Christopher D O Cooper, Tobias Krojer, James W. Murray, Ashley C W Pike, Apirat Chaikuad, Tracy Keates, Chancievan Thangaratnarajah, Viktorija Hojzan, Vikram Ayinampudi, Brian D. Marsden, Opher Gileadi, Stefan Knapp, Frank Von Delft, Alex N. Bullock

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 assembly, and substrate recognition into a single multidomain protein, of which the best known are BTB-BACK-Kelch domain proteins, including KEAP1. Cul3 assembly requires a BTB protein "3-box" motif, analogous to the F-box and SOCS box motifs of other Cullin-based E3s. To define the molecular basis for this assembly and the overall architecture of the E3, we determined the crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3, along with the Kelch domain structures of KLHL2 (Mayven), KLHL7, KLHL12, and KBTBD5. We show that Cul3 interaction is dependent on a unique N-terminal extension sequence that packs against the 3-box in a hydrophobic groove centrally located between the BTB and BACK domains. Deletion of this N-terminal region results in a 30-fold loss in affinity. The presented data offer a model for the quaternary assembly of this E3 class that supports the bivalent capture of Nrf2 and reveals potential new sites for E3 inhibitor design.

LanguageEnglish
Pages7803-7814
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number11
Early online date24 Jan 2013
DOIs
Publication statusPublished - 15 Mar 2013
Externally publishedYes

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Cullin Proteins
Ubiquitin-Protein Ligases
Ligases
Proteins
Ubiquitination
Substrates
Crystal structure
BTB-POZ Domain
Kelch Repeat

Cite this

Canning, P., Cooper, C. D. O., Krojer, T., Murray, J. W., Pike, A. C. W., Chaikuad, A., ... Bullock, A. N. (2013). Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases. Journal of Biological Chemistry, 288(11), 7803-7814. https://doi.org/10.1074/jbc.M112.437996
Canning, Peter ; Cooper, Christopher D O ; Krojer, Tobias ; Murray, James W. ; Pike, Ashley C W ; Chaikuad, Apirat ; Keates, Tracy ; Thangaratnarajah, Chancievan ; Hojzan, Viktorija ; Ayinampudi, Vikram ; Marsden, Brian D. ; Gileadi, Opher ; Knapp, Stefan ; Von Delft, Frank ; Bullock, Alex N. / Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 11. pp. 7803-7814.
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abstract = "Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 assembly, and substrate recognition into a single multidomain protein, of which the best known are BTB-BACK-Kelch domain proteins, including KEAP1. Cul3 assembly requires a BTB protein {"}3-box{"} motif, analogous to the F-box and SOCS box motifs of other Cullin-based E3s. To define the molecular basis for this assembly and the overall architecture of the E3, we determined the crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3, along with the Kelch domain structures of KLHL2 (Mayven), KLHL7, KLHL12, and KBTBD5. We show that Cul3 interaction is dependent on a unique N-terminal extension sequence that packs against the 3-box in a hydrophobic groove centrally located between the BTB and BACK domains. Deletion of this N-terminal region results in a 30-fold loss in affinity. The presented data offer a model for the quaternary assembly of this E3 class that supports the bivalent capture of Nrf2 and reveals potential new sites for E3 inhibitor design.",
author = "Peter Canning and Cooper, {Christopher D O} and Tobias Krojer and Murray, {James W.} and Pike, {Ashley C W} and Apirat Chaikuad and Tracy Keates and Chancievan Thangaratnarajah and Viktorija Hojzan and Vikram Ayinampudi and Marsden, {Brian D.} and Opher Gileadi and Stefan Knapp and {Von Delft}, Frank and Bullock, {Alex N.}",
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Canning, P, Cooper, CDO, Krojer, T, Murray, JW, Pike, ACW, Chaikuad, A, Keates, T, Thangaratnarajah, C, Hojzan, V, Ayinampudi, V, Marsden, BD, Gileadi, O, Knapp, S, Von Delft, F & Bullock, AN 2013, 'Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases', Journal of Biological Chemistry, vol. 288, no. 11, pp. 7803-7814. https://doi.org/10.1074/jbc.M112.437996

Structural Basis for Cul3 Protein Assembly with the BTB-Kelch Family of E3 Ubiquitin Ligases. / Canning, Peter; Cooper, Christopher D O; Krojer, Tobias; Murray, James W.; Pike, Ashley C W; Chaikuad, Apirat; Keates, Tracy; Thangaratnarajah, Chancievan; Hojzan, Viktorija; Ayinampudi, Vikram; Marsden, Brian D.; Gileadi, Opher; Knapp, Stefan; Von Delft, Frank; Bullock, Alex N.

In: Journal of Biological Chemistry, Vol. 288, No. 11, 15.03.2013, p. 7803-7814.

Research output: Contribution to journalArticle

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AU - Canning, Peter

AU - Cooper, Christopher D O

AU - Krojer, Tobias

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AU - Pike, Ashley C W

AU - Chaikuad, Apirat

AU - Keates, Tracy

AU - Thangaratnarajah, Chancievan

AU - Hojzan, Viktorija

AU - Ayinampudi, Vikram

AU - Marsden, Brian D.

AU - Gileadi, Opher

AU - Knapp, Stefan

AU - Von Delft, Frank

AU - Bullock, Alex N.

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N2 - Cullin-RING ligases are multisubunit E3 ubiquitin ligases that recruit substrate-specific adaptors to catalyze protein ubiquitylation. Cul3-based Cullin-RING ligases are uniquely associated with BTB adaptors that incorporate homodimerization, Cul3 assembly, and substrate recognition into a single multidomain protein, of which the best known are BTB-BACK-Kelch domain proteins, including KEAP1. Cul3 assembly requires a BTB protein "3-box" motif, analogous to the F-box and SOCS box motifs of other Cullin-based E3s. To define the molecular basis for this assembly and the overall architecture of the E3, we determined the crystal structures of the BTB-BACK domains of KLHL11 both alone and in complex with Cul3, along with the Kelch domain structures of KLHL2 (Mayven), KLHL7, KLHL12, and KBTBD5. We show that Cul3 interaction is dependent on a unique N-terminal extension sequence that packs against the 3-box in a hydrophobic groove centrally located between the BTB and BACK domains. Deletion of this N-terminal region results in a 30-fold loss in affinity. The presented data offer a model for the quaternary assembly of this E3 class that supports the bivalent capture of Nrf2 and reveals potential new sites for E3 inhibitor design.

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