Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach. & Thonn.)

E.i. Nep, S.m. Carnachan, N.c. Ngwuluka, V. Kontogiorgos, Gordon Morris, I.m. Sims, Alan Smith

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A polysaccharide from the leaves of Sesamum radiatum was extracted by maceration in deionized water followed by ethanol precipitation then chemically and physically characterised. Monosaccharide composition and linkages were determined by high performance anion exchange chromatography (HPAEC), gas chromatography–mass spectrometry (GC–MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. Sesamum gum was composed of glucuronic acid, mannose, galactose, and xylose with trace quantities of glucose, rhamnose and arabinose. Proton and 13C NMR spectroscopy, and linkage analysis revealed a glucuronomannan based structure comprising a backbone of →4)-β-D-GlcpA-(1 → 2)-α-D-Manp-(1→ with side-chains of galactose and xylose. Hydrated sesamum gum displayed temperature independent viscoelastic properties with no thermal hysteresis. Intrinsic viscosity was determined to be 3.31 and 4.40 dL g−1 in 0.1 M NaCl and deionised water respectively, while the critical concentration was determined to be 0.1% w/v. The characterisation performed in this study will help direct potential applications of this material in foods and pharmaceuticals.
LanguageEnglish
Pages541-547
Number of pages7
JournalCarbohydrate Polymers
Volume152
Early online date11 Jul 2016
DOIs
Publication statusPublished - 5 Nov 2016

Fingerprint

Xylose
Deionized water
Polysaccharides
Galactose
Nuclear magnetic resonance spectroscopy
Rhamnose
Glucuronic Acid
Arabinose
Monosaccharides
Mannose
Chromatography
Gas chromatography
Drug products
Anions
Mass spectrometry
Glucose
Hysteresis
Protons
Ion exchange
Ethanol

Cite this

@article{458fbbf8c7e540f38fa8647437c18b0f,
title = "Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach. & Thonn.)",
abstract = "A polysaccharide from the leaves of Sesamum radiatum was extracted by maceration in deionized water followed by ethanol precipitation then chemically and physically characterised. Monosaccharide composition and linkages were determined by high performance anion exchange chromatography (HPAEC), gas chromatography–mass spectrometry (GC–MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. Sesamum gum was composed of glucuronic acid, mannose, galactose, and xylose with trace quantities of glucose, rhamnose and arabinose. Proton and 13C NMR spectroscopy, and linkage analysis revealed a glucuronomannan based structure comprising a backbone of →4)-β-D-GlcpA-(1 → 2)-α-D-Manp-(1→ with side-chains of galactose and xylose. Hydrated sesamum gum displayed temperature independent viscoelastic properties with no thermal hysteresis. Intrinsic viscosity was determined to be 3.31 and 4.40 dL g−1 in 0.1 M NaCl and deionised water respectively, while the critical concentration was determined to be 0.1{\%} w/v. The characterisation performed in this study will help direct potential applications of this material in foods and pharmaceuticals.",
author = "E.i. Nep and S.m. Carnachan and N.c. Ngwuluka and V. Kontogiorgos and Gordon Morris and I.m. Sims and Alan Smith",
year = "2016",
month = "11",
day = "5",
doi = "10.1016/j.carbpol.2016.07.036",
language = "English",
volume = "152",
pages = "541--547",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",

}

Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach. & Thonn.). / Nep, E.i.; Carnachan, S.m.; Ngwuluka, N.c.; Kontogiorgos, V.; Morris, Gordon; Sims, I.m.; Smith, Alan.

In: Carbohydrate Polymers, Vol. 152, 05.11.2016, p. 541-547.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Structural characterisation and rheological properties of a polysaccharide from sesame leaves (Sesamum radiatum Schumach. & Thonn.)

AU - Nep, E.i.

AU - Carnachan, S.m.

AU - Ngwuluka, N.c.

AU - Kontogiorgos, V.

AU - Morris, Gordon

AU - Sims, I.m.

AU - Smith, Alan

PY - 2016/11/5

Y1 - 2016/11/5

N2 - A polysaccharide from the leaves of Sesamum radiatum was extracted by maceration in deionized water followed by ethanol precipitation then chemically and physically characterised. Monosaccharide composition and linkages were determined by high performance anion exchange chromatography (HPAEC), gas chromatography–mass spectrometry (GC–MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. Sesamum gum was composed of glucuronic acid, mannose, galactose, and xylose with trace quantities of glucose, rhamnose and arabinose. Proton and 13C NMR spectroscopy, and linkage analysis revealed a glucuronomannan based structure comprising a backbone of →4)-β-D-GlcpA-(1 → 2)-α-D-Manp-(1→ with side-chains of galactose and xylose. Hydrated sesamum gum displayed temperature independent viscoelastic properties with no thermal hysteresis. Intrinsic viscosity was determined to be 3.31 and 4.40 dL g−1 in 0.1 M NaCl and deionised water respectively, while the critical concentration was determined to be 0.1% w/v. The characterisation performed in this study will help direct potential applications of this material in foods and pharmaceuticals.

AB - A polysaccharide from the leaves of Sesamum radiatum was extracted by maceration in deionized water followed by ethanol precipitation then chemically and physically characterised. Monosaccharide composition and linkages were determined by high performance anion exchange chromatography (HPAEC), gas chromatography–mass spectrometry (GC–MS) and nuclear magnetic resonance (NMR) spectroscopy respectively. Sesamum gum was composed of glucuronic acid, mannose, galactose, and xylose with trace quantities of glucose, rhamnose and arabinose. Proton and 13C NMR spectroscopy, and linkage analysis revealed a glucuronomannan based structure comprising a backbone of →4)-β-D-GlcpA-(1 → 2)-α-D-Manp-(1→ with side-chains of galactose and xylose. Hydrated sesamum gum displayed temperature independent viscoelastic properties with no thermal hysteresis. Intrinsic viscosity was determined to be 3.31 and 4.40 dL g−1 in 0.1 M NaCl and deionised water respectively, while the critical concentration was determined to be 0.1% w/v. The characterisation performed in this study will help direct potential applications of this material in foods and pharmaceuticals.

U2 - 10.1016/j.carbpol.2016.07.036

DO - 10.1016/j.carbpol.2016.07.036

M3 - Article

VL - 152

SP - 541

EP - 547

JO - Carbohydrate Polymers

T2 - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -