Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23

L.P. Harding, V.M. Marshall, M. Elvin, Y. Gu, A.P. Laws

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:

A process for characterising polysaccharides having low solubility in aqueous solution is reported.

The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.
LanguageEnglish
Pages61-67
Number of pages7
JournalCarbohydrate Research
Volume338
Issue number1
DOIs
Publication statusPublished - 2 Jan 2003

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Lactobacillus delbrueckii
Nuclear magnetic resonance spectroscopy
Polysaccharides
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Sugars
Solubility
Monomers
octadecyl silane-bonded silica

Cite this

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title = "Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23",
abstract = "The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:A process for characterising polysaccharides having low solubility in aqueous solution is reported.The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.",
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T1 - Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy

T2 - Carbohydrate Research

AU - Harding, L.P.

AU - Marshall, V.M.

AU - Elvin, M.

AU - Gu, Y.

AU - Laws, A.P.

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AB - The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:A process for characterising polysaccharides having low solubility in aqueous solution is reported.The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.

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