Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23

L.P. Harding; V.M. Marshall; M. Elvin; Y. Gu; A.P. Laws

doi:10.1016/S0008-6215(02)00354-3

Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23

L.P. Harding, V.M. Marshall, M. Elvin, Y. Gu, A.P. Laws

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:

A process for characterising polysaccharides having low solubility in aqueous solution is reported.

The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.

Original language	English
Pages (from-to)	61-67
Number of pages	7
Journal	Carbohydrate Research
Volume	338
Issue number	1
DOIs	https://doi.org/10.1016/S0008-6215(02)00354-3
Publication status	Published - 2 Jan 2003

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title = "Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23",

abstract = "The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:A process for characterising polysaccharides having low solubility in aqueous solution is reported.The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.",

author = "L.P. Harding and V.M. Marshall and M. Elvin and Y. Gu and A.P. Laws",

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Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23. / Harding, L.P.; Marshall, V.M.; Elvin, M. et al.
In: Carbohydrate Research, Vol. 338, No. 1, 02.01.2003, p. 61-67.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy

T2 - Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23

AU - Harding, L.P.

AU - Marshall, V.M.

AU - Elvin, M.

AU - Gu, Y.

AU - Laws, A.P.

N1 - cited By 14

PY - 2003/1/2

Y1 - 2003/1/2

N2 - The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:A process for characterising polysaccharides having low solubility in aqueous solution is reported.The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.

AB - The exopolysaccharide (EPS) from Lactobacillus delbrueckii subsp. bulgaricus EU23 was perdeuteriomethylated and the perdeuteriomethylated EPS (pdm-EPS) purified by elution from a C18 Sep-Pak cartridge. Both 1D and 2D NMR spectra were recorded for the pdm-EPS and these were interpreted to provide assignments for the individual 1H and 13C resonances of the sugar residues of the repeating unit. Using a combination of the results from monomer analysis and linkage analysis of the native EPS and the ROESY and HMBC NMR spectra of the pdm-EPS the following structure has been determined for the repeating unit:A process for characterising polysaccharides having low solubility in aqueous solution is reported.The structure of the novel expolysaccharide from Lactobacillus delbrueckii subsp. bulgaricus EU23 was determined from the perdeuteriomethylated polysaccharide.

U2 - 10.1016/S0008-6215(02)00354-3

DO - 10.1016/S0008-6215(02)00354-3

M3 - Article

SN - 0008-6215

VL - 338

SP - 61

EP - 67

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 1

ER -

Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: Characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23

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