Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20

Valerie M. Marshall, Helen Dunn, Mark Elvin, Neil McLay, Yucheng Gu, Andrew P. Laws

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Streptococcus thermophilus EU20 when grown on skimmed milk secretes a high-molecular-weight exopolysaccharide that is composed of glucose, galactose and rhamnose in a molar ratio of 2:3:2. Using chemical techniques and 1D and 2D-NMR spectroscopy (1H and 13C) the polysaccharide has been shown to possess a heptasaccharide repeating unit having the following structure: Treatment of the polysaccharide with mild acid (0.5 M TFA, 100 °C for 1 h) liberates two oligosaccharides; the components correspond to the repeating unit and a hexasaccharide equivalent to the repeating unit minus the terminal α-L-Rhap.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalCarbohydrate Research
Issue number4
Publication statusPublished - 23 Apr 2001


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