Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20

Valerie M. Marshall, Helen Dunn, Mark Elvin, Neil McLay, Yucheng Gu, Andrew P. Laws

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Streptococcus thermophilus EU20 when grown on skimmed milk secretes a high-molecular-weight exopolysaccharide that is composed of glucose, galactose and rhamnose in a molar ratio of 2:3:2. Using chemical techniques and 1D and 2D-NMR spectroscopy (1H and 13C) the polysaccharide has been shown to possess a heptasaccharide repeating unit having the following structure: Treatment of the polysaccharide with mild acid (0.5 M TFA, 100 °C for 1 h) liberates two oligosaccharides; the components correspond to the repeating unit and a hexasaccharide equivalent to the repeating unit minus the terminal α-L-Rhap.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalCarbohydrate Research
Volume331
Issue number4
DOIs
Publication statusPublished - 23 Apr 2001

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Polysaccharides
Streptococcus thermophilus
Rhamnose
Oligosaccharides
Galactose
Nuclear magnetic resonance spectroscopy
Milk
Magnetic Resonance Spectroscopy
Molecular Weight
Molecular weight
Glucose
Acids
Streptococcus exopolysaccharide

Cite this

Marshall, Valerie M. ; Dunn, Helen ; Elvin, Mark ; McLay, Neil ; Gu, Yucheng ; Laws, Andrew P. / Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20. In: Carbohydrate Research. 2001 ; Vol. 331, No. 4. pp. 413-422.
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Structural characterisation of the exopolysaccharide produced by Streptococcus thermophilus EU20. / Marshall, Valerie M.; Dunn, Helen; Elvin, Mark; McLay, Neil; Gu, Yucheng; Laws, Andrew P.

In: Carbohydrate Research, Vol. 331, No. 4, 23.04.2001, p. 413-422.

Research output: Contribution to journalArticle

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AU - Laws, Andrew P.

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